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Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study
Isolated heavy sarcoplasmic reticulum vesicles retain junctional specializations (feet) on their outer surface. We have obtained en face three-dimensional views of the feet by shadowing and replicating the surfaces of freeze-dried isolated vesicles. Feet are clearly visible as large structures locat...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1984
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113360/ https://www.ncbi.nlm.nih.gov/pubmed/6386826 |
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collection | PubMed |
description | Isolated heavy sarcoplasmic reticulum vesicles retain junctional specializations (feet) on their outer surface. We have obtained en face three-dimensional views of the feet by shadowing and replicating the surfaces of freeze-dried isolated vesicles. Feet are clearly visible as large structures located on raised platforms. New details of foot structure include a four subunit structure and the fact that adjacent feet do not abut directly corner to corner but are offset by half a subunit. Feet aligned within rows were observed to be rotated at a slight angle off the long axis of the row creating a center-to-center spacing (32.5 nm) slightly less than the average diagonal of the feet (35.3 nm). Comparison with previous information from thin sections and freeze-fracture showed that this approach to the study of membranes faithfully preserves structure and allows better visualization of surface details than either thin-sectioning or negative-staining. |
format | Text |
id | pubmed-2113360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1984 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21133602008-05-01 Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study J Cell Biol Articles Isolated heavy sarcoplasmic reticulum vesicles retain junctional specializations (feet) on their outer surface. We have obtained en face three-dimensional views of the feet by shadowing and replicating the surfaces of freeze-dried isolated vesicles. Feet are clearly visible as large structures located on raised platforms. New details of foot structure include a four subunit structure and the fact that adjacent feet do not abut directly corner to corner but are offset by half a subunit. Feet aligned within rows were observed to be rotated at a slight angle off the long axis of the row creating a center-to-center spacing (32.5 nm) slightly less than the average diagonal of the feet (35.3 nm). Comparison with previous information from thin sections and freeze-fracture showed that this approach to the study of membranes faithfully preserves structure and allows better visualization of surface details than either thin-sectioning or negative-staining. The Rockefeller University Press 1984-11-01 /pmc/articles/PMC2113360/ /pubmed/6386826 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study |
title | Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study |
title_full | Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study |
title_fullStr | Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study |
title_full_unstemmed | Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study |
title_short | Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study |
title_sort | subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113360/ https://www.ncbi.nlm.nih.gov/pubmed/6386826 |