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Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide
The nucleotide sequence of a chicken genomic DNA segment containing the chicken beta 4 tubulin gene has been determined. The predicted amino acid sequence of beta 4 is surprisingly divergent from that of the chicken beta 2 gene that encodes the dominant neural beta tubulin. beta 4 differs from beta...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1984
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113369/ https://www.ncbi.nlm.nih.gov/pubmed/6490718 |
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collection | PubMed |
description | The nucleotide sequence of a chicken genomic DNA segment containing the chicken beta 4 tubulin gene has been determined. The predicted amino acid sequence of beta 4 is surprisingly divergent from that of the chicken beta 2 gene that encodes the dominant neural beta tubulin. beta 4 differs from beta 2 at 36 residue positions and encodes a polypeptide that is four amino acids longer, yielding a divergence of 8.9% between the two beta tubulin isotypes. While many of the amino acid substitutions are conservative, several involve significant alteration in the physiochemical properties of the residue. Furthermore, the amino acid substitution positions are not randomly located within the primary sequence but are distinctly clustered: major divergence occurs in the carboxy-terminal region beyond residue 430 and within the second protein coding exon segments of the genes. In addition, large regions of absolute sequence conservation are also present. Certain sequences within the heterogeneous regions are conserved in other species, indicating that these regions are under positive evolutionary selection pressure and are therefore probably essential for some aspect of beta- tubulin function. These findings strongly suggest that regional amino acid sequence heterogeneity may play an important role in the establishment of functionally differentiated beta tubulin polypeptides. |
format | Text |
id | pubmed-2113369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1984 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21133692008-05-01 Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide J Cell Biol Articles The nucleotide sequence of a chicken genomic DNA segment containing the chicken beta 4 tubulin gene has been determined. The predicted amino acid sequence of beta 4 is surprisingly divergent from that of the chicken beta 2 gene that encodes the dominant neural beta tubulin. beta 4 differs from beta 2 at 36 residue positions and encodes a polypeptide that is four amino acids longer, yielding a divergence of 8.9% between the two beta tubulin isotypes. While many of the amino acid substitutions are conservative, several involve significant alteration in the physiochemical properties of the residue. Furthermore, the amino acid substitution positions are not randomly located within the primary sequence but are distinctly clustered: major divergence occurs in the carboxy-terminal region beyond residue 430 and within the second protein coding exon segments of the genes. In addition, large regions of absolute sequence conservation are also present. Certain sequences within the heterogeneous regions are conserved in other species, indicating that these regions are under positive evolutionary selection pressure and are therefore probably essential for some aspect of beta- tubulin function. These findings strongly suggest that regional amino acid sequence heterogeneity may play an important role in the establishment of functionally differentiated beta tubulin polypeptides. The Rockefeller University Press 1984-11-01 /pmc/articles/PMC2113369/ /pubmed/6490718 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide |
title | Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide |
title_full | Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide |
title_fullStr | Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide |
title_full_unstemmed | Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide |
title_short | Sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide |
title_sort | sequence of a highly divergent beta tubulin gene reveals regional heterogeneity in the beta tubulin polypeptide |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113369/ https://www.ncbi.nlm.nih.gov/pubmed/6490718 |