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Experimental separation of pronuclei in fertilized sea urchin eggs: chromosomes do not organize a spindle in the absence of centrosomes
We tested the ability of chromosomes in a mitotic cytoplasm to organize a bipolar spindle in the absence of centrosomes. Sea urchin eggs were treated with 5 X 10(-6) colcemid for 7-9 min before fertilization to block future microtubule assembly. Fertilization events were normal except that a sperm a...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1985
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113528/ https://www.ncbi.nlm.nih.gov/pubmed/3972900 |