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Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate
We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpop...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1985
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113621/ https://www.ncbi.nlm.nih.gov/pubmed/3159736 |
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collection | PubMed |
description | We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded. |
format | Text |
id | pubmed-2113621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1985 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21136212008-05-01 Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate J Cell Biol Articles We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded. The Rockefeller University Press 1985-07-01 /pmc/articles/PMC2113621/ /pubmed/3159736 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate |
title | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate |
title_full | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate |
title_fullStr | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate |
title_full_unstemmed | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate |
title_short | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate |
title_sort | two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113621/ https://www.ncbi.nlm.nih.gov/pubmed/3159736 |