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Fast axonal transport of foreign synaptic vesicles in squid axoplasm

Translocation of intracellular organelles requires interaction with the cellular cytoskeleton, but the membrane and cytoskeletal proteins involved in movement are unknown. Here we show that highly purified synaptic vesicles from electric fish added to extruded squid axoplasm can show ATP-dependent m...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1985
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113654/
https://www.ncbi.nlm.nih.gov/pubmed/3848436
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description Translocation of intracellular organelles requires interaction with the cellular cytoskeleton, but the membrane and cytoskeletal proteins involved in movement are unknown. Here we show that highly purified synaptic vesicles from electric fish added to extruded squid axoplasm can show ATP-dependent movement. The movement is indistinguishable from that of endogenous vesicles and has a slight preference for the orthograde direction. In the presence of a nonhydrolyzable ATP analog, the synaptic vesicles bind to axoplasmic fibers but do not move. Elastase treatment of vesicles inhibits both binding and movement. We conclude that a protein component on the surface of cholinergic synaptic vesicles from electric fish is conserved during evolution and so can be recognized by the organelle-translocating machinery of the squid axon, resulting in ATP-dependent movement. Synaptic vesicles apparently retain the capacity for fast axonal transport, even after they reach their intracellular destination.
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spelling pubmed-21136542008-05-01 Fast axonal transport of foreign synaptic vesicles in squid axoplasm J Cell Biol Articles Translocation of intracellular organelles requires interaction with the cellular cytoskeleton, but the membrane and cytoskeletal proteins involved in movement are unknown. Here we show that highly purified synaptic vesicles from electric fish added to extruded squid axoplasm can show ATP-dependent movement. The movement is indistinguishable from that of endogenous vesicles and has a slight preference for the orthograde direction. In the presence of a nonhydrolyzable ATP analog, the synaptic vesicles bind to axoplasmic fibers but do not move. Elastase treatment of vesicles inhibits both binding and movement. We conclude that a protein component on the surface of cholinergic synaptic vesicles from electric fish is conserved during evolution and so can be recognized by the organelle-translocating machinery of the squid axon, resulting in ATP-dependent movement. Synaptic vesicles apparently retain the capacity for fast axonal transport, even after they reach their intracellular destination. The Rockefeller University Press 1985-08-01 /pmc/articles/PMC2113654/ /pubmed/3848436 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Fast axonal transport of foreign synaptic vesicles in squid axoplasm
title Fast axonal transport of foreign synaptic vesicles in squid axoplasm
title_full Fast axonal transport of foreign synaptic vesicles in squid axoplasm
title_fullStr Fast axonal transport of foreign synaptic vesicles in squid axoplasm
title_full_unstemmed Fast axonal transport of foreign synaptic vesicles in squid axoplasm
title_short Fast axonal transport of foreign synaptic vesicles in squid axoplasm
title_sort fast axonal transport of foreign synaptic vesicles in squid axoplasm
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113654/
https://www.ncbi.nlm.nih.gov/pubmed/3848436