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Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells

Several types of cells store proteins in secretory vesicles from which they are released by an appropriate stimulus. It might be expected that the secretory vesicles in different cell types use similar molecular machinery. Here we describe a transmembrane glycoprotein (Mr approximately 100,000) that...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1985
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113776/
https://www.ncbi.nlm.nih.gov/pubmed/2579958
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description Several types of cells store proteins in secretory vesicles from which they are released by an appropriate stimulus. It might be expected that the secretory vesicles in different cell types use similar molecular machinery. Here we describe a transmembrane glycoprotein (Mr approximately 100,000) that is present in secretory vesicles in all neurons and endocrine cells studied, in species from elasmobranch fish to mammals, and in neural and endocrine cell lines. It was detected by cross-reactivity with monoclonal antibodies raised to highly purified cholinergic synaptic vesicles from the electric organ of fish. By immunoprecipitation of intact synaptic vesicles and electron microscopic immunoperoxidase labeling, we have shown that the antigenic determinant is on the cytoplasmic face of the synaptic vesicles. However, the electrophoretic mobility of the antigen synthesized in the presence of tunicamycin is reduced to Mr approximately 62,000, which suggests that the antigen is glycosylated and must therefore span the vesicle membrane.
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spelling pubmed-21137762008-05-01 Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells J Cell Biol Articles Several types of cells store proteins in secretory vesicles from which they are released by an appropriate stimulus. It might be expected that the secretory vesicles in different cell types use similar molecular machinery. Here we describe a transmembrane glycoprotein (Mr approximately 100,000) that is present in secretory vesicles in all neurons and endocrine cells studied, in species from elasmobranch fish to mammals, and in neural and endocrine cell lines. It was detected by cross-reactivity with monoclonal antibodies raised to highly purified cholinergic synaptic vesicles from the electric organ of fish. By immunoprecipitation of intact synaptic vesicles and electron microscopic immunoperoxidase labeling, we have shown that the antigenic determinant is on the cytoplasmic face of the synaptic vesicles. However, the electrophoretic mobility of the antigen synthesized in the presence of tunicamycin is reduced to Mr approximately 62,000, which suggests that the antigen is glycosylated and must therefore span the vesicle membrane. The Rockefeller University Press 1985-04-01 /pmc/articles/PMC2113776/ /pubmed/2579958 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells
title Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells
title_full Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells
title_fullStr Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells
title_full_unstemmed Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells
title_short Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells
title_sort identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113776/
https://www.ncbi.nlm.nih.gov/pubmed/2579958