Molecular cloning of cDNA for rat liver gap junction protein

An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used t...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1986
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113807/
https://www.ncbi.nlm.nih.gov/pubmed/3013898
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collection PubMed
description An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used to select cDNA from a rat liver library in the expression vector lambda gt11. The largest cDNA selected contained 1,494 bp and coded for a protein with a calculated molecular mass of 32,007 daltons. Northern blot analysis indicated that brain, kidney, and stomach express an mRNA with similar size and homology to that expressed in liver, but that heart and lens express differently sized, less homologous mRNA.
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spelling pubmed-21138072008-05-01 Molecular cloning of cDNA for rat liver gap junction protein J Cell Biol Articles An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used to select cDNA from a rat liver library in the expression vector lambda gt11. The largest cDNA selected contained 1,494 bp and coded for a protein with a calculated molecular mass of 32,007 daltons. Northern blot analysis indicated that brain, kidney, and stomach express an mRNA with similar size and homology to that expressed in liver, but that heart and lens express differently sized, less homologous mRNA. The Rockefeller University Press 1986-07-01 /pmc/articles/PMC2113807/ /pubmed/3013898 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Molecular cloning of cDNA for rat liver gap junction protein
title Molecular cloning of cDNA for rat liver gap junction protein
title_full Molecular cloning of cDNA for rat liver gap junction protein
title_fullStr Molecular cloning of cDNA for rat liver gap junction protein
title_full_unstemmed Molecular cloning of cDNA for rat liver gap junction protein
title_short Molecular cloning of cDNA for rat liver gap junction protein
title_sort molecular cloning of cdna for rat liver gap junction protein
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113807/
https://www.ncbi.nlm.nih.gov/pubmed/3013898

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