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Active maturation-promoting factor is present in mature mouse oocytes
Cytoplasmic extracts of meiotically mature mouse oocytes were injected into immature Xenopus laevis oocytes, which underwent germinal vesicle breakdown within 2 h. Germinal vesicle breakdown was not inhibited by incubation of the Xenopus oocytes in cycloheximide (20 micrograms/ml). Identically prepa...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1985
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113872/ https://www.ncbi.nlm.nih.gov/pubmed/3886670 |
| _version_ | 1782140287022268416 |
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| collection | PubMed |
| description | Cytoplasmic extracts of meiotically mature mouse oocytes were injected into immature Xenopus laevis oocytes, which underwent germinal vesicle breakdown within 2 h. Germinal vesicle breakdown was not inhibited by incubation of the Xenopus oocytes in cycloheximide (20 micrograms/ml). Identically prepared extracts of meiotically immature mouse oocytes, arrested at the germinal vesicle stage by dibutyryl cyclic AMP (100 micrograms/ml), did not induce germinal vesicle breakdown in Xenopus oocytes. The results show that maturation-promoting factor activity appears during the course of oocyte maturation in the mouse. |
| format | Text |
| id | pubmed-2113872 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1985 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21138722008-05-01 Active maturation-promoting factor is present in mature mouse oocytes J Cell Biol Articles Cytoplasmic extracts of meiotically mature mouse oocytes were injected into immature Xenopus laevis oocytes, which underwent germinal vesicle breakdown within 2 h. Germinal vesicle breakdown was not inhibited by incubation of the Xenopus oocytes in cycloheximide (20 micrograms/ml). Identically prepared extracts of meiotically immature mouse oocytes, arrested at the germinal vesicle stage by dibutyryl cyclic AMP (100 micrograms/ml), did not induce germinal vesicle breakdown in Xenopus oocytes. The results show that maturation-promoting factor activity appears during the course of oocyte maturation in the mouse. The Rockefeller University Press 1985-05-01 /pmc/articles/PMC2113872/ /pubmed/3886670 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles Active maturation-promoting factor is present in mature mouse oocytes |
| title | Active maturation-promoting factor is present in mature mouse oocytes |
| title_full | Active maturation-promoting factor is present in mature mouse oocytes |
| title_fullStr | Active maturation-promoting factor is present in mature mouse oocytes |
| title_full_unstemmed | Active maturation-promoting factor is present in mature mouse oocytes |
| title_short | Active maturation-promoting factor is present in mature mouse oocytes |
| title_sort | active maturation-promoting factor is present in mature mouse oocytes |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113872/ https://www.ncbi.nlm.nih.gov/pubmed/3886670 |