Cargando…
Characterization of maturation-dependent extrinsic proteins of the rat sperm surface
Mammalian spermatozoa must mature in the epididymis before they can fertilize an egg. It is known that modification of the protein composition of the sperm surface is an important part of the maturation process. In this paper, we present data on two related glycoproteins that can be extracted from m...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1985
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113883/ https://www.ncbi.nlm.nih.gov/pubmed/3886668 |
_version_ | 1782140289637416960 |
---|---|
collection | PubMed |
description | Mammalian spermatozoa must mature in the epididymis before they can fertilize an egg. It is known that modification of the protein composition of the sperm surface is an important part of the maturation process. In this paper, we present data on two related glycoproteins that can be extracted from mature but not immature spermatozoa. Cell surface radioiodination has shown that these proteins are on the sperm surface, and immunofluorescence microscopy, by use of monospecific antibodies to the proteins, has indicated that their localization is restricted to the periacrosomal region of the sperm head. We have also shown that in vitro, these proteins will bind to the identical region of immature sperm. Immunohistochemical localization of the proteins in the epididymis shows that they are produced and secreted by the cauda region. The significance of the addition of these proteins to the sperm surface in both maturation and fertilization is discussed. |
format | Text |
id | pubmed-2113883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1985 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21138832008-05-01 Characterization of maturation-dependent extrinsic proteins of the rat sperm surface J Cell Biol Articles Mammalian spermatozoa must mature in the epididymis before they can fertilize an egg. It is known that modification of the protein composition of the sperm surface is an important part of the maturation process. In this paper, we present data on two related glycoproteins that can be extracted from mature but not immature spermatozoa. Cell surface radioiodination has shown that these proteins are on the sperm surface, and immunofluorescence microscopy, by use of monospecific antibodies to the proteins, has indicated that their localization is restricted to the periacrosomal region of the sperm head. We have also shown that in vitro, these proteins will bind to the identical region of immature sperm. Immunohistochemical localization of the proteins in the epididymis shows that they are produced and secreted by the cauda region. The significance of the addition of these proteins to the sperm surface in both maturation and fertilization is discussed. The Rockefeller University Press 1985-05-01 /pmc/articles/PMC2113883/ /pubmed/3886668 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Characterization of maturation-dependent extrinsic proteins of the rat sperm surface |
title | Characterization of maturation-dependent extrinsic proteins of the rat sperm surface |
title_full | Characterization of maturation-dependent extrinsic proteins of the rat sperm surface |
title_fullStr | Characterization of maturation-dependent extrinsic proteins of the rat sperm surface |
title_full_unstemmed | Characterization of maturation-dependent extrinsic proteins of the rat sperm surface |
title_short | Characterization of maturation-dependent extrinsic proteins of the rat sperm surface |
title_sort | characterization of maturation-dependent extrinsic proteins of the rat sperm surface |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113883/ https://www.ncbi.nlm.nih.gov/pubmed/3886668 |