Cargando…

Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells

Newly synthesized G protein of vesicular stomatitis virus is not transported to the surface of cultured mammalian cells during mitosis (Warren et al., 1983, J. Cell Biol. 97:1623-1628). To determine where intracellular transport is inhibited, we have examined the post- translational modifications of...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1985
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114025/
https://www.ncbi.nlm.nih.gov/pubmed/2999158
_version_ 1782140322915024896
collection PubMed
description Newly synthesized G protein of vesicular stomatitis virus is not transported to the surface of cultured mammalian cells during mitosis (Warren et al., 1983, J. Cell Biol. 97:1623-1628). To determine where intracellular transport is inhibited, we have examined the post- translational modifications of G protein, which are indicators of specific compartments on the transport pathway. G protein in mitotic cells had only endo H-sensitive oligosaccharides containing seven or eight mannose residues, but no terminal glucose, and was not fatty acylated. These modifications were indicative of processing only by enzymes of the endoplasmic reticulum (ER). Quantitative immunocytochemistry was used as an independent method to confirm that transport of G protein out of the ER was inhibited. The density of G protein in the ER cisternae was 2.5 times greater than in infected G1 cells treated similarly. Incubation of infected mitotic cells with cycloheximide, which inhibits protein synthesis without affecting transport, did not result in a decrease in the density of G protein in the ER cisternae, demonstrating that G protein cannot be chased out of the ER. These results suggest that intracellular transport stops at or before the first vesicle-mediated step on the pathway.
format Text
id pubmed-2114025
institution National Center for Biotechnology Information
language English
publishDate 1985
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21140252008-05-01 Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells J Cell Biol Articles Newly synthesized G protein of vesicular stomatitis virus is not transported to the surface of cultured mammalian cells during mitosis (Warren et al., 1983, J. Cell Biol. 97:1623-1628). To determine where intracellular transport is inhibited, we have examined the post- translational modifications of G protein, which are indicators of specific compartments on the transport pathway. G protein in mitotic cells had only endo H-sensitive oligosaccharides containing seven or eight mannose residues, but no terminal glucose, and was not fatty acylated. These modifications were indicative of processing only by enzymes of the endoplasmic reticulum (ER). Quantitative immunocytochemistry was used as an independent method to confirm that transport of G protein out of the ER was inhibited. The density of G protein in the ER cisternae was 2.5 times greater than in infected G1 cells treated similarly. Incubation of infected mitotic cells with cycloheximide, which inhibits protein synthesis without affecting transport, did not result in a decrease in the density of G protein in the ER cisternae, demonstrating that G protein cannot be chased out of the ER. These results suggest that intracellular transport stops at or before the first vesicle-mediated step on the pathway. The Rockefeller University Press 1985-12-01 /pmc/articles/PMC2114025/ /pubmed/2999158 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells
title Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells
title_full Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells
title_fullStr Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells
title_full_unstemmed Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells
title_short Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells
title_sort newly synthesized g protein of vesicular stomatitis virus is not transported to the golgi complex in mitotic cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114025/
https://www.ncbi.nlm.nih.gov/pubmed/2999158