Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes

The accompanying report (Watanabe, M., A. L. Frelinger III, and U. Rutishauser, 1986, J. Cell Biol., 103:1721-1727) describes a set of monoclonal antibodies (mAbs) directed against N-CAM epitopes representing the known major structural and functional domains of the molecule. In this study, we have g...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1986
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114365/
https://www.ncbi.nlm.nih.gov/pubmed/2430977
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collection PubMed
description The accompanying report (Watanabe, M., A. L. Frelinger III, and U. Rutishauser, 1986, J. Cell Biol., 103:1721-1727) describes a set of monoclonal antibodies (mAbs) directed against N-CAM epitopes representing the known major structural and functional domains of the molecule. In this study, we have generated and separated a variety of peptide fragments from N-CAM, and then used their size and reactivity with each antibody to position the antigenic sites along the peptide chain. This epitope map, together with the biological properties of the antibodies and previous studies on N-CAM, have been used to construct a topographical model for the molecule in the cell membrane.
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spelling pubmed-21143652008-05-01 Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes J Cell Biol Articles The accompanying report (Watanabe, M., A. L. Frelinger III, and U. Rutishauser, 1986, J. Cell Biol., 103:1721-1727) describes a set of monoclonal antibodies (mAbs) directed against N-CAM epitopes representing the known major structural and functional domains of the molecule. In this study, we have generated and separated a variety of peptide fragments from N-CAM, and then used their size and reactivity with each antibody to position the antigenic sites along the peptide chain. This epitope map, together with the biological properties of the antibodies and previous studies on N-CAM, have been used to construct a topographical model for the molecule in the cell membrane. The Rockefeller University Press 1986-11-01 /pmc/articles/PMC2114365/ /pubmed/2430977 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes
title Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes
title_full Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes
title_fullStr Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes
title_full_unstemmed Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes
title_short Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes
title_sort topography of n-cam structural and functional determinants. ii. placement of monoclonal antibody epitopes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114365/
https://www.ncbi.nlm.nih.gov/pubmed/2430977

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