Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins

Temperature-sensitive mutations in the RNA2 through RNA11 genes of yeast prevent the processing of nuclear pre-mRNAs. We have raised antisera that detect the RNA2 and RNA3 proteins in immunoblots of extracts of yeast containing high copy number RNA2 and RNA3 plasmids. Subcellular fractionation of ye...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1986
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114587/
https://www.ncbi.nlm.nih.gov/pubmed/3536958
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collection PubMed
description Temperature-sensitive mutations in the RNA2 through RNA11 genes of yeast prevent the processing of nuclear pre-mRNAs. We have raised antisera that detect the RNA2 and RNA3 proteins in immunoblots of extracts of yeast containing high copy number RNA2 and RNA3 plasmids. Subcellular fractionation of yeast cells that overproduce the RNA2 and RNA3 proteins has revealed that these proteins are enriched in nuclear fractions. Indirect immunofluorescence results have indicated that these proteins are localized in yeast nuclei. These localization results are consistent with the fact that these genes have a role in processing yeast pre-mRNA.
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spelling pubmed-21145872008-05-01 Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins J Cell Biol Articles Temperature-sensitive mutations in the RNA2 through RNA11 genes of yeast prevent the processing of nuclear pre-mRNAs. We have raised antisera that detect the RNA2 and RNA3 proteins in immunoblots of extracts of yeast containing high copy number RNA2 and RNA3 plasmids. Subcellular fractionation of yeast cells that overproduce the RNA2 and RNA3 proteins has revealed that these proteins are enriched in nuclear fractions. Indirect immunofluorescence results have indicated that these proteins are localized in yeast nuclei. These localization results are consistent with the fact that these genes have a role in processing yeast pre-mRNA. The Rockefeller University Press 1986-12-01 /pmc/articles/PMC2114587/ /pubmed/3536958 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins
title Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins
title_full Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins
title_fullStr Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins
title_full_unstemmed Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins
title_short Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins
title_sort identification and nuclear localization of yeast pre-messenger rna processing components: rna2 and rna3 proteins
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114587/
https://www.ncbi.nlm.nih.gov/pubmed/3536958

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