An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides

We examined the actin-nucleating activity in polymorphonuclear leukocyte lysates prepared at various times after chemotactic peptide addition. The actin nucleation increases two- to threefold within 15 s after peptide addition, decays to basal levels within 90 s, and is largely independent of cytopl...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1986
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114615/
https://www.ncbi.nlm.nih.gov/pubmed/3793753
_version_ 1782140461468614656
collection PubMed
description We examined the actin-nucleating activity in polymorphonuclear leukocyte lysates prepared at various times after chemotactic peptide addition. The actin nucleation increases two- to threefold within 15 s after peptide addition, decays to basal levels within 90 s, and is largely independent of cytoplasmic calcium fluxes. The peptide-induced nucleation sites behave as free barbed ends and therefore may increase the level of polymerized actin in vivo. The new nucleation sites may also determine the cellular sites of actin polymerization. This localization of actin polymerization could be important for the directional extension of lamellipodia during chemotaxis.
format Text
id pubmed-2114615
institution National Center for Biotechnology Information
language English
publishDate 1986
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21146152008-05-01 An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides J Cell Biol Articles We examined the actin-nucleating activity in polymorphonuclear leukocyte lysates prepared at various times after chemotactic peptide addition. The actin nucleation increases two- to threefold within 15 s after peptide addition, decays to basal levels within 90 s, and is largely independent of cytoplasmic calcium fluxes. The peptide-induced nucleation sites behave as free barbed ends and therefore may increase the level of polymerized actin in vivo. The new nucleation sites may also determine the cellular sites of actin polymerization. This localization of actin polymerization could be important for the directional extension of lamellipodia during chemotaxis. The Rockefeller University Press 1986-12-01 /pmc/articles/PMC2114615/ /pubmed/3793753 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides
title An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides
title_full An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides
title_fullStr An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides
title_full_unstemmed An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides
title_short An actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides
title_sort actin-nucleating activity in polymorphonuclear leukocytes is modulated by chemotactic peptides
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114615/
https://www.ncbi.nlm.nih.gov/pubmed/3793753

Ejemplares similares