Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor

We have found that a soluble activity present in the postribosomal supernatant fraction of Saccharomyces cerevisiae stimulates posttranslational translocation of yeast prepro-alpha-factor across yeast microsomal membranes. Stimulation of translocation is not due to a nonspecific affect on ATP levels...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1986
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114630/
https://www.ncbi.nlm.nih.gov/pubmed/3025220
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collection PubMed
description We have found that a soluble activity present in the postribosomal supernatant fraction of Saccharomyces cerevisiae stimulates posttranslational translocation of yeast prepro-alpha-factor across yeast microsomal membranes. Stimulation of translocation is not due to a nonspecific affect on ATP levels. The activity is likely to be due to protein(s) as it is destroyed by N-ethylmaleimide, protease, or heat treatment but not by incubation with RNase. Its apparent sedimentation coefficient is approximately 9.6 S.
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spelling pubmed-21146302008-05-01 Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor J Cell Biol Articles We have found that a soluble activity present in the postribosomal supernatant fraction of Saccharomyces cerevisiae stimulates posttranslational translocation of yeast prepro-alpha-factor across yeast microsomal membranes. Stimulation of translocation is not due to a nonspecific affect on ATP levels. The activity is likely to be due to protein(s) as it is destroyed by N-ethylmaleimide, protease, or heat treatment but not by incubation with RNase. Its apparent sedimentation coefficient is approximately 9.6 S. The Rockefeller University Press 1986-12-01 /pmc/articles/PMC2114630/ /pubmed/3025220 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
title Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
title_full Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
title_fullStr Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
title_full_unstemmed Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
title_short Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
title_sort protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114630/
https://www.ncbi.nlm.nih.gov/pubmed/3025220

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