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The mechanism of osmotic transfection of avian embryonic erythrocytes: analysis of a system for studying developmental gene expression
We have undertaken a study of the mechanism of DNA transfer into primary chicken erythrocytes by a method named osmotic transfection. The cells are subjected to controlled osmotic swelling in NH4Cl and then ruptured in a lower osmotic strength solution containing DNA and DEAE-dextran. The osmotic ru...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1987
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114811/ https://www.ncbi.nlm.nih.gov/pubmed/3477547 |