Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes

We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recogni...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1987
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114812/
https://www.ncbi.nlm.nih.gov/pubmed/3308902
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collection PubMed
description We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recognize antigens on the lateral transcription loops are described here. We suggest that these antigens are proteins associated with nascent RNA transcripts, although they are not among the 30-40-kD "core" heterogeneous nuclear ribonucleoproteins.
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spelling pubmed-21148122008-05-01 Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes J Cell Biol Articles We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recognize antigens on the lateral transcription loops are described here. We suggest that these antigens are proteins associated with nascent RNA transcripts, although they are not among the 30-40-kD "core" heterogeneous nuclear ribonucleoproteins. The Rockefeller University Press 1987-09-01 /pmc/articles/PMC2114812/ /pubmed/3308902 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes
title Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes
title_full Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes
title_fullStr Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes
title_full_unstemmed Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes
title_short Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes
title_sort monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114812/
https://www.ncbi.nlm.nih.gov/pubmed/3308902

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