Cargando…

Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies

Laminin derived from the Engelbreth-Holm-Swarm (EHS) tumor and a lamininlike molecule synthesized by RN22 Schwannoma cells both stimulate rapid neurite outgrowth, consistent with a common neurite- promoting site. However, antilaminin antisera can only inhibit the activity of the EHS laminin. The blo...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114995/
https://www.ncbi.nlm.nih.gov/pubmed/2452171
_version_ 1782140552184070144
collection PubMed
description Laminin derived from the Engelbreth-Holm-Swarm (EHS) tumor and a lamininlike molecule synthesized by RN22 Schwannoma cells both stimulate rapid neurite outgrowth, consistent with a common neurite- promoting site. However, antilaminin antisera can only inhibit the activity of the EHS laminin. The blocking antibodies in such sera are directed against the terminal heparin-binding domain of the laminin long arm (Edgar, D., R. Timpl, and H. Thoenen. 1984. EMBO [Eur. Mol. Biol. Organ.] J. 3: 1463-1468). These epitopes are demonstrated by immunoblotting to be part of the A chain and to be absent in RN22 laminin, showing (through metabolic labeling) that the cells synthesized little if any 440-kD A chain. This indicates that the antibody inhibition was probably due to steric hindrance, a common neurite-promoting site, apparently not being antigenic in native molecules. Antibodies raised against a 25-kD proteolytic fragment derived from the long arm of laminin were then used as probes to identify other potential neurite-promoting structures. Although these antibodies do not cross-react with native laminin, they recognized the B chains of denatured EHS and RN22 molecules on immunoblots. The antibodies also bound to the large proteolytic fragment, derived from the long arm of laminin that contains the neurite-promoting site, thus inhibiting its activity. Taken together, these results point to the localization of normally nonantigenic, defined, B chain sequences within or close to the neurite-promoting site of laminin.
format Text
id pubmed-2114995
institution National Center for Biotechnology Information
language English
publishDate 1988
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21149952008-05-01 Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies J Cell Biol Articles Laminin derived from the Engelbreth-Holm-Swarm (EHS) tumor and a lamininlike molecule synthesized by RN22 Schwannoma cells both stimulate rapid neurite outgrowth, consistent with a common neurite- promoting site. However, antilaminin antisera can only inhibit the activity of the EHS laminin. The blocking antibodies in such sera are directed against the terminal heparin-binding domain of the laminin long arm (Edgar, D., R. Timpl, and H. Thoenen. 1984. EMBO [Eur. Mol. Biol. Organ.] J. 3: 1463-1468). These epitopes are demonstrated by immunoblotting to be part of the A chain and to be absent in RN22 laminin, showing (through metabolic labeling) that the cells synthesized little if any 440-kD A chain. This indicates that the antibody inhibition was probably due to steric hindrance, a common neurite-promoting site, apparently not being antigenic in native molecules. Antibodies raised against a 25-kD proteolytic fragment derived from the long arm of laminin were then used as probes to identify other potential neurite-promoting structures. Although these antibodies do not cross-react with native laminin, they recognized the B chains of denatured EHS and RN22 molecules on immunoblots. The antibodies also bound to the large proteolytic fragment, derived from the long arm of laminin that contains the neurite-promoting site, thus inhibiting its activity. Taken together, these results point to the localization of normally nonantigenic, defined, B chain sequences within or close to the neurite-promoting site of laminin. The Rockefeller University Press 1988-04-01 /pmc/articles/PMC2114995/ /pubmed/2452171 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies
title Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies
title_full Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies
title_fullStr Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies
title_full_unstemmed Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies
title_short Structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies
title_sort structural requirements for the stimulation of neurite outgrowth by two variants of laminin and their inhibition by antibodies
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114995/
https://www.ncbi.nlm.nih.gov/pubmed/2452171