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Differential regulation of Paramecium ciliary motility by cAMP and cGMP

cAMP and cGMP had distinct effects on the regulation of ciliary motility in Paramecium. Using detergent-permeabilized cells reactivated to swim with MgATP, we observed effects of cyclic nucleotides and interactions with Ca2+ on the swimming speed and direction of reactivated cells. Both cAMP and cGM...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115034/
https://www.ncbi.nlm.nih.gov/pubmed/2836435
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description cAMP and cGMP had distinct effects on the regulation of ciliary motility in Paramecium. Using detergent-permeabilized cells reactivated to swim with MgATP, we observed effects of cyclic nucleotides and interactions with Ca2+ on the swimming speed and direction of reactivated cells. Both cAMP and cGMP increased forward swimming speed two- to threefold with similar half-maximal concentrations near 0.5 microM. The two cyclic nucleotides, however, had different effects in antagonism with the Ca2+ response of backward swimming and on the handedness of the helical swimming paths of reactivated cells. These results suggest that cAMP and cGMP differentially regulate the direction of the ciliary power stroke.
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spelling pubmed-21150342008-05-01 Differential regulation of Paramecium ciliary motility by cAMP and cGMP J Cell Biol Articles cAMP and cGMP had distinct effects on the regulation of ciliary motility in Paramecium. Using detergent-permeabilized cells reactivated to swim with MgATP, we observed effects of cyclic nucleotides and interactions with Ca2+ on the swimming speed and direction of reactivated cells. Both cAMP and cGMP increased forward swimming speed two- to threefold with similar half-maximal concentrations near 0.5 microM. The two cyclic nucleotides, however, had different effects in antagonism with the Ca2+ response of backward swimming and on the handedness of the helical swimming paths of reactivated cells. These results suggest that cAMP and cGMP differentially regulate the direction of the ciliary power stroke. The Rockefeller University Press 1988-05-01 /pmc/articles/PMC2115034/ /pubmed/2836435 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Differential regulation of Paramecium ciliary motility by cAMP and cGMP
title Differential regulation of Paramecium ciliary motility by cAMP and cGMP
title_full Differential regulation of Paramecium ciliary motility by cAMP and cGMP
title_fullStr Differential regulation of Paramecium ciliary motility by cAMP and cGMP
title_full_unstemmed Differential regulation of Paramecium ciliary motility by cAMP and cGMP
title_short Differential regulation of Paramecium ciliary motility by cAMP and cGMP
title_sort differential regulation of paramecium ciliary motility by camp and cgmp
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115034/
https://www.ncbi.nlm.nih.gov/pubmed/2836435