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Organization of microtubules in centrosome-free cytoplasm

Many different cell types possess microtubule patterns which appear to be polarized and oriented, in part, by cytoplasmic factors not directly associated with a centrosome. Recently, we demonstrated that cytoplasmic extensions ("arms") of teleost melanophores will reorganize their microtub...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115052/
https://www.ncbi.nlm.nih.gov/pubmed/3286659
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description Many different cell types possess microtubule patterns which appear to be polarized and oriented, in part, by cytoplasmic factors not directly associated with a centrosome. Recently, we demonstrated that cytoplasmic extensions ("arms") of teleost melanophores will reorganize their microtubule population outward from their centers after surgical isolation (McNiven, M. A., M. Wang, and K. R. Porter. 1984. Cell. 37:753-765). In the study reported here, we examine microtubule dynamics within the centrosome-free fragments and find that, after severing, microtubule reorganization is initiated at the proximal (cut) end of an arm and migrates distally with the aggregated pigment mass until it becomes permanently positioned at the middle of the arm. Computer-aided image analysis demonstrates that this middle position is located at the arm centroid, implicating the action of a cytoplasmic gel in this process. Morphological studies of arms devoid of pigment reveal that microtubules do not emanate from a single site or structure within the centroid area, but from a more generalized region. Taken together, these findings suggest that factors distributed throughout cytoplasm participate in microtubule assembly and organization.
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spelling pubmed-21150522008-05-01 Organization of microtubules in centrosome-free cytoplasm J Cell Biol Articles Many different cell types possess microtubule patterns which appear to be polarized and oriented, in part, by cytoplasmic factors not directly associated with a centrosome. Recently, we demonstrated that cytoplasmic extensions ("arms") of teleost melanophores will reorganize their microtubule population outward from their centers after surgical isolation (McNiven, M. A., M. Wang, and K. R. Porter. 1984. Cell. 37:753-765). In the study reported here, we examine microtubule dynamics within the centrosome-free fragments and find that, after severing, microtubule reorganization is initiated at the proximal (cut) end of an arm and migrates distally with the aggregated pigment mass until it becomes permanently positioned at the middle of the arm. Computer-aided image analysis demonstrates that this middle position is located at the arm centroid, implicating the action of a cytoplasmic gel in this process. Morphological studies of arms devoid of pigment reveal that microtubules do not emanate from a single site or structure within the centroid area, but from a more generalized region. Taken together, these findings suggest that factors distributed throughout cytoplasm participate in microtubule assembly and organization. The Rockefeller University Press 1988-05-01 /pmc/articles/PMC2115052/ /pubmed/3286659 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Organization of microtubules in centrosome-free cytoplasm
title Organization of microtubules in centrosome-free cytoplasm
title_full Organization of microtubules in centrosome-free cytoplasm
title_fullStr Organization of microtubules in centrosome-free cytoplasm
title_full_unstemmed Organization of microtubules in centrosome-free cytoplasm
title_short Organization of microtubules in centrosome-free cytoplasm
title_sort organization of microtubules in centrosome-free cytoplasm
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115052/
https://www.ncbi.nlm.nih.gov/pubmed/3286659