Cargando…

A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation

The conversion of determined adipoblasts to fully differentiated adipocytes requires appropriate environmental conditions. A strict dependence on cell confluence and a facilitation by glucocorticoid hormones have previously been described. We have found that agents that are capable of activating pro...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115191/
https://www.ncbi.nlm.nih.gov/pubmed/2455724
_version_ 1782140598464020480
collection PubMed
description The conversion of determined adipoblasts to fully differentiated adipocytes requires appropriate environmental conditions. A strict dependence on cell confluence and a facilitation by glucocorticoid hormones have previously been described. We have found that agents that are capable of activating protein kinase C, such as basic fibroblast growth factor and phorbol esters, inhibit the differentiation of the adipogenic cell line TA1 without stimulating cell growth. Here we describe the sequence and characterization of a cDNA (clone 5) that detects an RNA, the expression of which is enhanced by glucocorticoids and increasing cell density. In contrast, activators of protein kinase C including basic fibroblast growth factor, phorbol esters, and synthetic diacylglycerols inhibit clone 5 gene expression. It appears that clone 5 expression is closely linked to environmental and hormonal factors that promote the differentiation of adipogenic cells.
format Text
id pubmed-2115191
institution National Center for Biotechnology Information
language English
publishDate 1988
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21151912008-05-01 A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation J Cell Biol Articles The conversion of determined adipoblasts to fully differentiated adipocytes requires appropriate environmental conditions. A strict dependence on cell confluence and a facilitation by glucocorticoid hormones have previously been described. We have found that agents that are capable of activating protein kinase C, such as basic fibroblast growth factor and phorbol esters, inhibit the differentiation of the adipogenic cell line TA1 without stimulating cell growth. Here we describe the sequence and characterization of a cDNA (clone 5) that detects an RNA, the expression of which is enhanced by glucocorticoids and increasing cell density. In contrast, activators of protein kinase C including basic fibroblast growth factor, phorbol esters, and synthetic diacylglycerols inhibit clone 5 gene expression. It appears that clone 5 expression is closely linked to environmental and hormonal factors that promote the differentiation of adipogenic cells. The Rockefeller University Press 1988-07-01 /pmc/articles/PMC2115191/ /pubmed/2455724 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation
title A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation
title_full A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation
title_fullStr A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation
title_full_unstemmed A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation
title_short A growth factor-repressible gene associated with protein kinase C- mediated inhibition of adipocyte differentiation
title_sort growth factor-repressible gene associated with protein kinase c- mediated inhibition of adipocyte differentiation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115191/
https://www.ncbi.nlm.nih.gov/pubmed/2455724