Cargando…

Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells

The coding region of the hamster desmin gene was fused to the 5' flanking sequences of the hamster vimentin gene and introduced into the germ line of mice. The expression of this intermediate filament gene construct (pVDes) was analyzed at the RNA and protein level in transgenic mice as well as...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115373/
https://www.ncbi.nlm.nih.gov/pubmed/2646305
_version_ 1782140641055080448
collection PubMed
description The coding region of the hamster desmin gene was fused to the 5' flanking sequences of the hamster vimentin gene and introduced into the germ line of mice. The expression of this intermediate filament gene construct (pVDes) was analyzed at the RNA and protein level in transgenic mice as well as in fibroblast cell lines and primary hepatocyte cultures derived from these mice. In all transgenic mice, the pVDes-encoded protein was coexpressed with mouse vimentin in a tissue-specific fashion and was indistinguishable from normal hamster desmin. Culturing of transgenic hepatocytes induced desmin expression indicating that 3.2 kbp of the vimentin gene 5' region regulates both tissue-specific and tissue culture-induced intermediate filament protein expression. Immunohistochemical staining and double-label immunoelectron microscopy of cultured transgenic fibroblasts showed that the pVDes protein assembled into intermediate filaments which colocalized with the mouse vimentin filaments. Endogenous vimentin RNA levels were not influenced by high-level pVDes expression. The coexpression of desmin and vimentin in nonmuscle cells did not result in detectable developmental, morphological, or physiological abnormalities.
format Text
id pubmed-2115373
institution National Center for Biotechnology Information
language English
publishDate 1989
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21153732008-05-01 Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells J Cell Biol Articles The coding region of the hamster desmin gene was fused to the 5' flanking sequences of the hamster vimentin gene and introduced into the germ line of mice. The expression of this intermediate filament gene construct (pVDes) was analyzed at the RNA and protein level in transgenic mice as well as in fibroblast cell lines and primary hepatocyte cultures derived from these mice. In all transgenic mice, the pVDes-encoded protein was coexpressed with mouse vimentin in a tissue-specific fashion and was indistinguishable from normal hamster desmin. Culturing of transgenic hepatocytes induced desmin expression indicating that 3.2 kbp of the vimentin gene 5' region regulates both tissue-specific and tissue culture-induced intermediate filament protein expression. Immunohistochemical staining and double-label immunoelectron microscopy of cultured transgenic fibroblasts showed that the pVDes protein assembled into intermediate filaments which colocalized with the mouse vimentin filaments. Endogenous vimentin RNA levels were not influenced by high-level pVDes expression. The coexpression of desmin and vimentin in nonmuscle cells did not result in detectable developmental, morphological, or physiological abnormalities. The Rockefeller University Press 1989-03-01 /pmc/articles/PMC2115373/ /pubmed/2646305 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells
title Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells
title_full Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells
title_fullStr Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells
title_full_unstemmed Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells
title_short Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells
title_sort transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115373/
https://www.ncbi.nlm.nih.gov/pubmed/2646305