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Access of proteinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes
We have used proteinase K as a probe to detect cytoplasmically and luminally exposed segments of nascent polypeptides undergoing transport across mammalian microsomal membranes. A series of translocation intermediates consisting of discrete-sized nascent chains was prepared by including microsomal m...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1989
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115410/ https://www.ncbi.nlm.nih.gov/pubmed/2537313 |