Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin

We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha- actinin. This specific isoform of...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115443/
https://www.ncbi.nlm.nih.gov/pubmed/2645301
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collection PubMed
description We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha- actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch- specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha- actinin.
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spelling pubmed-21154432008-05-01 Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin J Cell Biol Articles We have characterized a protein immunologically related to dystrophin, the protein product of the Duchenne muscular dystrophy gene. We identify this related protein as a fast-twitch glycolytic isoform (mouse extensor digitorum longus-specific) of myofibrillar alpha- actinin. This specific isoform of alpha-actinin exhibits a more restricted pattern of expression in skeletal muscle than fast-twitch- specific isoforms of both myosin and Ca2+-ATPase. Our results provide evidence that dystrophin and myofibrillar alpha-actinin are related proteins, reinforcing the previous data concerning the sequence homologies noted between nonmuscle cytoskeletal alpha-actinin and dystrophin. In addition, we describe the first antisera directed against a specific myofibrillar skeletal muscle isoform of alpha- actinin. The Rockefeller University Press 1989-02-01 /pmc/articles/PMC2115443/ /pubmed/2645301 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin
title Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin
title_full Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin
title_fullStr Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin
title_full_unstemmed Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin
title_short Detection of a specific isoform of alpha-actinin with antisera directed against dystrophin
title_sort detection of a specific isoform of alpha-actinin with antisera directed against dystrophin
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115443/
https://www.ncbi.nlm.nih.gov/pubmed/2645301

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