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In vitro membrane assembly of a polytopic, transmembrane protein results in an enzymatically active conformation
In vitro integration of the polytopic, transmembrane lactose permease into membrane vesicles from Escherichia coli is demonstrated. To this end the enzyme was synthesized in a homologous, cell-free transcription- translation system. In this system, synthesis occurred in an essentially membrane-free...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1989
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115569/ https://www.ncbi.nlm.nih.gov/pubmed/2654138 |