Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones

We have purified a polyclonal antibody by affinity chromatography which binds specifically to the phosphorylated form of the regulatory light chain (Mr = 20,000) of smooth muscle myosin. This antibody does not stain relaxed, permeabilized smooth muscle cells isolated from guinea pig taenia coli. How...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115625/
https://www.ncbi.nlm.nih.gov/pubmed/3060469
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collection PubMed
description We have purified a polyclonal antibody by affinity chromatography which binds specifically to the phosphorylated form of the regulatory light chain (Mr = 20,000) of smooth muscle myosin. This antibody does not stain relaxed, permeabilized smooth muscle cells isolated from guinea pig taenia coli. However, when these cells were stimulated to contract with CaCl2 (100 microM) and ATP (1 mM), the immunofluorescence staining was localized in a series of transverse bands. This distribution of activated myosin appears to reflect an underlying structural organization of the smooth muscle cell cytoskeleton into mechanically coupled contractile zones.
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spelling pubmed-21156252008-05-01 Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones J Cell Biol Articles We have purified a polyclonal antibody by affinity chromatography which binds specifically to the phosphorylated form of the regulatory light chain (Mr = 20,000) of smooth muscle myosin. This antibody does not stain relaxed, permeabilized smooth muscle cells isolated from guinea pig taenia coli. However, when these cells were stimulated to contract with CaCl2 (100 microM) and ATP (1 mM), the immunofluorescence staining was localized in a series of transverse bands. This distribution of activated myosin appears to reflect an underlying structural organization of the smooth muscle cell cytoskeleton into mechanically coupled contractile zones. The Rockefeller University Press 1988-12-01 /pmc/articles/PMC2115625/ /pubmed/3060469 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones
title Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones
title_full Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones
title_fullStr Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones
title_full_unstemmed Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones
title_short Spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones
title_sort spatial pattern of myosin phosphorylation in contracting smooth muscle cells: evidence for contractile zones
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115625/
https://www.ncbi.nlm.nih.gov/pubmed/3060469

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