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The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable
An internal 1.4-kb Bst EII fragment was used to disrupt the benA gene and establish heterokaryons. The heterokaryons demonstrated that the molecular disruption of benA results in a recessive benA null mutation. Conidia from a heterokaryon swell and germinate but cannot undergo nuclear division and a...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1989
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115864/ https://www.ncbi.nlm.nih.gov/pubmed/2681229 |
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collection | PubMed |
description | An internal 1.4-kb Bst EII fragment was used to disrupt the benA gene and establish heterokaryons. The heterokaryons demonstrated that the molecular disruption of benA results in a recessive benA null mutation. Conidia from a heterokaryon swell and germinate but cannot undergo nuclear division and are thus inviable. A chimeric beta-tubulin gene was constructed with the benA promoter driving the tubC structural gene. This chimeric gene construction was placed on a plasmid containing a selectable marker for Aspergillus transformation and the gene disrupting fragment of benA. Integration of this plasmid at benA by the internal gene disrupting fragment of benA simultaneously disrupts the benA gene and replaces it with the chimeric beta-tubulin gene, rescuing the benA null generated by the integration. Strains generated by this procedure contain only tubC beta-tubulin for all beta- tubulin functions. Strains having only tubC beta-tubulin are viable and exhibit no detectable microtubule dysfunction though they are more sensitive than wild-type strains to the antimicrotubule drug benomyl. It is concluded that the two beta-tubulin genes of Aspergillus nidulans, though highly divergent, are interchangeable. |
format | Text |
id | pubmed-2115864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1989 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21158642008-05-01 The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable J Cell Biol Articles An internal 1.4-kb Bst EII fragment was used to disrupt the benA gene and establish heterokaryons. The heterokaryons demonstrated that the molecular disruption of benA results in a recessive benA null mutation. Conidia from a heterokaryon swell and germinate but cannot undergo nuclear division and are thus inviable. A chimeric beta-tubulin gene was constructed with the benA promoter driving the tubC structural gene. This chimeric gene construction was placed on a plasmid containing a selectable marker for Aspergillus transformation and the gene disrupting fragment of benA. Integration of this plasmid at benA by the internal gene disrupting fragment of benA simultaneously disrupts the benA gene and replaces it with the chimeric beta-tubulin gene, rescuing the benA null generated by the integration. Strains generated by this procedure contain only tubC beta-tubulin for all beta- tubulin functions. Strains having only tubC beta-tubulin are viable and exhibit no detectable microtubule dysfunction though they are more sensitive than wild-type strains to the antimicrotubule drug benomyl. It is concluded that the two beta-tubulin genes of Aspergillus nidulans, though highly divergent, are interchangeable. The Rockefeller University Press 1989-11-01 /pmc/articles/PMC2115864/ /pubmed/2681229 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable |
title | The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable |
title_full | The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable |
title_fullStr | The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable |
title_full_unstemmed | The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable |
title_short | The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable |
title_sort | highly divergent beta-tubulins of aspergillus nidulans are functionally interchangeable |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115864/ https://www.ncbi.nlm.nih.gov/pubmed/2681229 |