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Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells
We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. M...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115986/ https://www.ncbi.nlm.nih.gov/pubmed/2404021 |
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collection | PubMed |
description | We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Muller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions. |
format | Text |
id | pubmed-2115986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21159862008-05-01 Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells J Cell Biol Articles We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Muller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions. The Rockefeller University Press 1990-01-01 /pmc/articles/PMC2115986/ /pubmed/2404021 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells |
title | Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells |
title_full | Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells |
title_fullStr | Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells |
title_full_unstemmed | Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells |
title_short | Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells |
title_sort | purification of profilin from saccharomyces cerevisiae and analysis of profilin-deficient cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115986/ https://www.ncbi.nlm.nih.gov/pubmed/2404021 |