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The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation
So far it has been demonstrated that the signal sequence of proteins which are made at the ER functions both at the level of protein targeting to the ER and in initiation of chain translocation across the ER membrane. However, its possible role in completing the process of chain transfer (see Singer...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116283/ https://www.ncbi.nlm.nih.gov/pubmed/2391367 |
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collection | PubMed |
description | So far it has been demonstrated that the signal sequence of proteins which are made at the ER functions both at the level of protein targeting to the ER and in initiation of chain translocation across the ER membrane. However, its possible role in completing the process of chain transfer (see Singer, S. J., P. A. Maher, and M. P. Yaffe. Proc. Natl. Acad. Sci. USA. 1987. 84:1015-1019) has remained elusive. In this work we show that the p62 protein of Semliki Forest virus contains an uncleaved signal sequence at its NH2-terminus and that this becomes glycosylated early during synthesis and translocation of the p62 polypeptide. As the glycosylation of the signal sequence most likely occurs after its release from the ER membrane our results suggest that this region has no role in completing the transfer process. |
format | Text |
id | pubmed-2116283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21162832008-05-01 The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation J Cell Biol Articles So far it has been demonstrated that the signal sequence of proteins which are made at the ER functions both at the level of protein targeting to the ER and in initiation of chain translocation across the ER membrane. However, its possible role in completing the process of chain transfer (see Singer, S. J., P. A. Maher, and M. P. Yaffe. Proc. Natl. Acad. Sci. USA. 1987. 84:1015-1019) has remained elusive. In this work we show that the p62 protein of Semliki Forest virus contains an uncleaved signal sequence at its NH2-terminus and that this becomes glycosylated early during synthesis and translocation of the p62 polypeptide. As the glycosylation of the signal sequence most likely occurs after its release from the ER membrane our results suggest that this region has no role in completing the transfer process. The Rockefeller University Press 1990-09-01 /pmc/articles/PMC2116283/ /pubmed/2391367 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation |
title | The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation |
title_full | The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation |
title_fullStr | The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation |
title_full_unstemmed | The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation |
title_short | The signal sequence of the p62 protein of Semliki Forest virus is involved in initiation but not in completing chain translocation |
title_sort | signal sequence of the p62 protein of semliki forest virus is involved in initiation but not in completing chain translocation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116283/ https://www.ncbi.nlm.nih.gov/pubmed/2391367 |