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Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure

We have studied the expression of fibulin in cultured fibroblasts and determined its primary structure by cDNA cloning. Our results show that fibulin is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix when expressed by cultured cells or added exogenously to ce...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116371/
https://www.ncbi.nlm.nih.gov/pubmed/2269669
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description We have studied the expression of fibulin in cultured fibroblasts and determined its primary structure by cDNA cloning. Our results show that fibulin is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix when expressed by cultured cells or added exogenously to cell monolayers. In addition, we find that fibulin is present in plasma at a level of 33 +/- 3 micrograms/ml. Sequencing of multiple fibulin cDNAs indicates that a process of alternative splicing results in the expression of three fibulin transcripts. The transcripts encode overlapping polypeptides differing only in carboxy- terminal segments. Common to the three predicted forms of fibulin is a unique 537-amino acid-long cysteine-rich polypeptide and a 29-residue signal peptide. The amino-terminal portion of fibulin contains a repeated element with potential disulfide loop structure resembling that of the complement component anaphylatoxins C3a, C4a, and C5a as well as proteins of the albumin gene family. The bulk of the remaining portion of the molecule is a series of nine EGF-like repeats.
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spelling pubmed-21163712008-05-01 Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure J Cell Biol Articles We have studied the expression of fibulin in cultured fibroblasts and determined its primary structure by cDNA cloning. Our results show that fibulin is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix when expressed by cultured cells or added exogenously to cell monolayers. In addition, we find that fibulin is present in plasma at a level of 33 +/- 3 micrograms/ml. Sequencing of multiple fibulin cDNAs indicates that a process of alternative splicing results in the expression of three fibulin transcripts. The transcripts encode overlapping polypeptides differing only in carboxy- terminal segments. Common to the three predicted forms of fibulin is a unique 537-amino acid-long cysteine-rich polypeptide and a 29-residue signal peptide. The amino-terminal portion of fibulin contains a repeated element with potential disulfide loop structure resembling that of the complement component anaphylatoxins C3a, C4a, and C5a as well as proteins of the albumin gene family. The bulk of the remaining portion of the molecule is a series of nine EGF-like repeats. The Rockefeller University Press 1990-12-01 /pmc/articles/PMC2116371/ /pubmed/2269669 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure
title Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure
title_full Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure
title_fullStr Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure
title_full_unstemmed Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure
title_short Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure
title_sort fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116371/
https://www.ncbi.nlm.nih.gov/pubmed/2269669