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Immunocytology with microwave-fixed fibroblasts shows 1 alpha,25- dihydroxyvitamin D3-dependent rapid and estrogen-dependent slow reorganization of vitamin D receptors
Prior studies have given no evidence for regulation of vitamin D receptor (VDR) compartmentalization or subcellular organization. Microwave fixation (9-15 s) and an indirect immunodetection system of avidin-biotin enhancement and phycoerythrin fluorophore resulted in sufficient spatial and temporal...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1990
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116405/ https://www.ncbi.nlm.nih.gov/pubmed/2177476 |