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The fate of human Langerhans cells in hematopoietic stem cell transplantation
Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118090/ https://www.ncbi.nlm.nih.gov/pubmed/16390938 http://dx.doi.org/10.1084/jem.20051787 |
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author | Collin, Matthew P. Hart, Derek N.J. Jackson, Graham H. Cook, Gordon Cavet, James Mackinnon, Stephen Middleton, Peter G. Dickinson, Anne M. |
author_facet | Collin, Matthew P. Hart, Derek N.J. Jackson, Graham H. Cook, Gordon Cavet, James Mackinnon, Stephen Middleton, Peter G. Dickinson, Anne M. |
author_sort | Collin, Matthew P. |
collection | PubMed |
description | Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant. Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14–21 d. Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active. LC chimerism was determined in sex-mismatched transplants using a two-step Giemsa/fluorescence in situ hybridization assay on isolated cells. Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment. At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%. Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment. |
format | Text |
id | pubmed-2118090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21180902007-12-13 The fate of human Langerhans cells in hematopoietic stem cell transplantation Collin, Matthew P. Hart, Derek N.J. Jackson, Graham H. Cook, Gordon Cavet, James Mackinnon, Stephen Middleton, Peter G. Dickinson, Anne M. J Exp Med Brief Definitive Reports Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant. Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14–21 d. Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active. LC chimerism was determined in sex-mismatched transplants using a two-step Giemsa/fluorescence in situ hybridization assay on isolated cells. Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment. At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%. Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment. The Rockefeller University Press 2006-01-23 /pmc/articles/PMC2118090/ /pubmed/16390938 http://dx.doi.org/10.1084/jem.20051787 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Brief Definitive Reports Collin, Matthew P. Hart, Derek N.J. Jackson, Graham H. Cook, Gordon Cavet, James Mackinnon, Stephen Middleton, Peter G. Dickinson, Anne M. The fate of human Langerhans cells in hematopoietic stem cell transplantation |
title | The fate of human Langerhans cells in hematopoietic stem cell transplantation |
title_full | The fate of human Langerhans cells in hematopoietic stem cell transplantation |
title_fullStr | The fate of human Langerhans cells in hematopoietic stem cell transplantation |
title_full_unstemmed | The fate of human Langerhans cells in hematopoietic stem cell transplantation |
title_short | The fate of human Langerhans cells in hematopoietic stem cell transplantation |
title_sort | fate of human langerhans cells in hematopoietic stem cell transplantation |
topic | Brief Definitive Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118090/ https://www.ncbi.nlm.nih.gov/pubmed/16390938 http://dx.doi.org/10.1084/jem.20051787 |
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