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Induction of a virus-specific effector–memory CD4(+) T cell response by attenuated SIV infection

We investigated simian immunodeficiency virus (SIV)-specific CD4(+) T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVΔnef-infected animals revealed a relatively high frequency of SIV-specific CD4(+) T cells representing 4–10% of all C...

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Detalles Bibliográficos
Autores principales: Gauduin, Marie-Claire, Yu, Yi, Barabasz, Amy, Carville, Angela, Piatak, Mike, Lifson, Jeffrey D., Desrosiers, Ronald C., Johnson, R. Paul
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118155/
https://www.ncbi.nlm.nih.gov/pubmed/17116733
http://dx.doi.org/10.1084/jem.20060134
Descripción
Sumario:We investigated simian immunodeficiency virus (SIV)-specific CD4(+) T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVΔnef-infected animals revealed a relatively high frequency of SIV-specific CD4(+) T cells representing 4–10% of all CD4(+) T lymphocytes directed against multiple SIV proteins. Gag-specific CD4(+) T cells in wild-type SIV-infected animals were 5–10-fold lower in frequency and inversely correlated with the level of plasma viremia. SIV-specific CD4(+) cells from SIVΔnef animals were predominantly CD27(−)CD28(−)CD45RA(low)CCR7(−)CCR5(−), consistent with an effector–memory subset, and included a fully differentiated CD45RA(+)CCR7(−) subpopulation. In contrast, SIV-specific CD4(+) T cells from SIV-infected animals were mostly CD27(+)CD28(+)CD45RA(−)CCR7(+)CCR5(+), consistent with an early central memory phenotype. The CD45RA(+)CCR7(−)CD4(+) subset from SIVΔnef animals was highly enriched for effector CD4(+) T cells, as indicated by the perforin expression and up-regulation of the lysosomal membrane protein CD107a after SIV Gag stimulation. SIV-specific CD4(+) T cells in attenuated SIV-infected animals were increased in frequency in bronchioalveolar lavage and decreased in lymph nodes, consistent with an effector–memory T cell population. The ability of SIVΔnef to induce a high frequency virus-specific CD4(+) T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains.