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Induction of a virus-specific effector–memory CD4(+) T cell response by attenuated SIV infection
We investigated simian immunodeficiency virus (SIV)-specific CD4(+) T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVΔnef-infected animals revealed a relatively high frequency of SIV-specific CD4(+) T cells representing 4–10% of all C...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118155/ https://www.ncbi.nlm.nih.gov/pubmed/17116733 http://dx.doi.org/10.1084/jem.20060134 |
Sumario: | We investigated simian immunodeficiency virus (SIV)-specific CD4(+) T cell responses in rhesus macaques chronically infected with attenuated or pathogenic SIV strains. Analysis of SIVΔnef-infected animals revealed a relatively high frequency of SIV-specific CD4(+) T cells representing 4–10% of all CD4(+) T lymphocytes directed against multiple SIV proteins. Gag-specific CD4(+) T cells in wild-type SIV-infected animals were 5–10-fold lower in frequency and inversely correlated with the level of plasma viremia. SIV-specific CD4(+) cells from SIVΔnef animals were predominantly CD27(−)CD28(−)CD45RA(low)CCR7(−)CCR5(−), consistent with an effector–memory subset, and included a fully differentiated CD45RA(+)CCR7(−) subpopulation. In contrast, SIV-specific CD4(+) T cells from SIV-infected animals were mostly CD27(+)CD28(+)CD45RA(−)CCR7(+)CCR5(+), consistent with an early central memory phenotype. The CD45RA(+)CCR7(−)CD4(+) subset from SIVΔnef animals was highly enriched for effector CD4(+) T cells, as indicated by the perforin expression and up-regulation of the lysosomal membrane protein CD107a after SIV Gag stimulation. SIV-specific CD4(+) T cells in attenuated SIV-infected animals were increased in frequency in bronchioalveolar lavage and decreased in lymph nodes, consistent with an effector–memory T cell population. The ability of SIVΔnef to induce a high frequency virus-specific CD4(+) T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains. |
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