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Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement

Cells lining the gastrointestinal tract serve as both a barrier to and a pathway for infectious agent entry. Dendritic cells (DCs) present in the lamina propria under the columnar villus epithelium of the small bowel extend processes across this epithelium and capture bacteria, but previous studies...

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Detalles Bibliográficos
Autores principales: Chieppa, Marcello, Rescigno, Maria, Huang, Alex Y.C., Germain, Ronald N.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118178/
https://www.ncbi.nlm.nih.gov/pubmed/17145958
http://dx.doi.org/10.1084/jem.20061884
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author Chieppa, Marcello
Rescigno, Maria
Huang, Alex Y.C.
Germain, Ronald N.
author_facet Chieppa, Marcello
Rescigno, Maria
Huang, Alex Y.C.
Germain, Ronald N.
author_sort Chieppa, Marcello
collection PubMed
description Cells lining the gastrointestinal tract serve as both a barrier to and a pathway for infectious agent entry. Dendritic cells (DCs) present in the lamina propria under the columnar villus epithelium of the small bowel extend processes across this epithelium and capture bacteria, but previous studies provided limited information on the nature of the stimuli, receptors, and signaling events involved in promoting this phenomenon. Here, we use immunohistochemical as well as dynamic explant and intravital two-photon imaging to investigate this issue. Analysis of CD11c–enhanced green fluorescent protein (EGFP) or major histocompatibility complex CII-EGFP mice revealed that the number of trans-epithelial DC extensions, many with an unusual “balloon” shape, varies along the length of the small bowel. High numbers of such extensions were found in the proximal jejunum, but only a few were present in the terminal ileum. The extensions in the terminal ileum markedly increased upon the introduction of invasive or noninvasive Salmonella organisms, and chimeric mouse studies revealed the key role of MyD88-dependent Toll-like receptor (TLR) signaling by nonhematopoietic (epithelial) elements in the DC extension response. Collectively, these findings support a model in which epithelial cell TLR signaling upon exposure to microbial stimuli induces active DC sampling of the gut lumen at sites distant from organized lymphoid tissues.
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spelling pubmed-21181782007-12-13 Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement Chieppa, Marcello Rescigno, Maria Huang, Alex Y.C. Germain, Ronald N. J Exp Med Articles Cells lining the gastrointestinal tract serve as both a barrier to and a pathway for infectious agent entry. Dendritic cells (DCs) present in the lamina propria under the columnar villus epithelium of the small bowel extend processes across this epithelium and capture bacteria, but previous studies provided limited information on the nature of the stimuli, receptors, and signaling events involved in promoting this phenomenon. Here, we use immunohistochemical as well as dynamic explant and intravital two-photon imaging to investigate this issue. Analysis of CD11c–enhanced green fluorescent protein (EGFP) or major histocompatibility complex CII-EGFP mice revealed that the number of trans-epithelial DC extensions, many with an unusual “balloon” shape, varies along the length of the small bowel. High numbers of such extensions were found in the proximal jejunum, but only a few were present in the terminal ileum. The extensions in the terminal ileum markedly increased upon the introduction of invasive or noninvasive Salmonella organisms, and chimeric mouse studies revealed the key role of MyD88-dependent Toll-like receptor (TLR) signaling by nonhematopoietic (epithelial) elements in the DC extension response. Collectively, these findings support a model in which epithelial cell TLR signaling upon exposure to microbial stimuli induces active DC sampling of the gut lumen at sites distant from organized lymphoid tissues. The Rockefeller University Press 2006-12-25 /pmc/articles/PMC2118178/ /pubmed/17145958 http://dx.doi.org/10.1084/jem.20061884 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Chieppa, Marcello
Rescigno, Maria
Huang, Alex Y.C.
Germain, Ronald N.
Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement
title Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement
title_full Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement
title_fullStr Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement
title_full_unstemmed Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement
title_short Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement
title_sort dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell tlr engagement
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118178/
https://www.ncbi.nlm.nih.gov/pubmed/17145958
http://dx.doi.org/10.1084/jem.20061884
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