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The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells

It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra- as well as Tcra-targeting experiments have suggested that the early expression of Tcra in CD4(−)CD8(−) c...

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Autores principales: Aifantis, Iannis, Bassing, Craig H., Garbe, Annette I., Sawai, Katie, Alt, Frederick W., von Boehmer, Harald
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118313/
https://www.ncbi.nlm.nih.gov/pubmed/16754716
http://dx.doi.org/10.1084/jem.20051711
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author Aifantis, Iannis
Bassing, Craig H.
Garbe, Annette I.
Sawai, Katie
Alt, Frederick W.
von Boehmer, Harald
author_facet Aifantis, Iannis
Bassing, Craig H.
Garbe, Annette I.
Sawai, Katie
Alt, Frederick W.
von Boehmer, Harald
author_sort Aifantis, Iannis
collection PubMed
description It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra- as well as Tcra-targeting experiments have suggested that the early expression of Tcra in CD4(−)CD8(−) cells can partially rescue the development of αβ CD4(+)CD8(+) cells in Ptcra-deficient mice. In this study, we show that the TCR Eδ but not Eα enhancer function is required for the cell surface expression of αβTCR on immature CD4(−)CD8(−) T cell precursors, which play a crucial role in promoting αβ T cell development in the absence of pre-TCR. Thus, αβTCR expression by CD4(−)CD8(−) thymocytes not only represents a transgenic artifact but occurs under physiological conditions.
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spelling pubmed-21183132007-12-13 The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells Aifantis, Iannis Bassing, Craig H. Garbe, Annette I. Sawai, Katie Alt, Frederick W. von Boehmer, Harald J Exp Med Articles It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra- as well as Tcra-targeting experiments have suggested that the early expression of Tcra in CD4(−)CD8(−) cells can partially rescue the development of αβ CD4(+)CD8(+) cells in Ptcra-deficient mice. In this study, we show that the TCR Eδ but not Eα enhancer function is required for the cell surface expression of αβTCR on immature CD4(−)CD8(−) T cell precursors, which play a crucial role in promoting αβ T cell development in the absence of pre-TCR. Thus, αβTCR expression by CD4(−)CD8(−) thymocytes not only represents a transgenic artifact but occurs under physiological conditions. The Rockefeller University Press 2006-06-12 /pmc/articles/PMC2118313/ /pubmed/16754716 http://dx.doi.org/10.1084/jem.20051711 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Aifantis, Iannis
Bassing, Craig H.
Garbe, Annette I.
Sawai, Katie
Alt, Frederick W.
von Boehmer, Harald
The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells
title The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells
title_full The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells
title_fullStr The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells
title_full_unstemmed The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells
title_short The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells
title_sort eδ enhancer controls the generation of cd4(−)cd8(−) αβtcr-expressing t cells that can give rise to different lineages of αβ t cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118313/
https://www.ncbi.nlm.nih.gov/pubmed/16754716
http://dx.doi.org/10.1084/jem.20051711
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