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The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells
It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra- as well as Tcra-targeting experiments have suggested that the early expression of Tcra in CD4(−)CD8(−) c...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118313/ https://www.ncbi.nlm.nih.gov/pubmed/16754716 http://dx.doi.org/10.1084/jem.20051711 |
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author | Aifantis, Iannis Bassing, Craig H. Garbe, Annette I. Sawai, Katie Alt, Frederick W. von Boehmer, Harald |
author_facet | Aifantis, Iannis Bassing, Craig H. Garbe, Annette I. Sawai, Katie Alt, Frederick W. von Boehmer, Harald |
author_sort | Aifantis, Iannis |
collection | PubMed |
description | It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra- as well as Tcra-targeting experiments have suggested that the early expression of Tcra in CD4(−)CD8(−) cells can partially rescue the development of αβ CD4(+)CD8(+) cells in Ptcra-deficient mice. In this study, we show that the TCR Eδ but not Eα enhancer function is required for the cell surface expression of αβTCR on immature CD4(−)CD8(−) T cell precursors, which play a crucial role in promoting αβ T cell development in the absence of pre-TCR. Thus, αβTCR expression by CD4(−)CD8(−) thymocytes not only represents a transgenic artifact but occurs under physiological conditions. |
format | Text |
id | pubmed-2118313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21183132007-12-13 The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells Aifantis, Iannis Bassing, Craig H. Garbe, Annette I. Sawai, Katie Alt, Frederick W. von Boehmer, Harald J Exp Med Articles It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra- as well as Tcra-targeting experiments have suggested that the early expression of Tcra in CD4(−)CD8(−) cells can partially rescue the development of αβ CD4(+)CD8(+) cells in Ptcra-deficient mice. In this study, we show that the TCR Eδ but not Eα enhancer function is required for the cell surface expression of αβTCR on immature CD4(−)CD8(−) T cell precursors, which play a crucial role in promoting αβ T cell development in the absence of pre-TCR. Thus, αβTCR expression by CD4(−)CD8(−) thymocytes not only represents a transgenic artifact but occurs under physiological conditions. The Rockefeller University Press 2006-06-12 /pmc/articles/PMC2118313/ /pubmed/16754716 http://dx.doi.org/10.1084/jem.20051711 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Aifantis, Iannis Bassing, Craig H. Garbe, Annette I. Sawai, Katie Alt, Frederick W. von Boehmer, Harald The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells |
title | The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells |
title_full | The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells |
title_fullStr | The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells |
title_full_unstemmed | The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells |
title_short | The Eδ enhancer controls the generation of CD4(−)CD8(−) αβTCR-expressing T cells that can give rise to different lineages of αβ T cells |
title_sort | eδ enhancer controls the generation of cd4(−)cd8(−) αβtcr-expressing t cells that can give rise to different lineages of αβ t cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118313/ https://www.ncbi.nlm.nih.gov/pubmed/16754716 http://dx.doi.org/10.1084/jem.20051711 |
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