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Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread

The biological relevance of nonneutralizing antibodies elicited early after infection with noncytopathic persistence-prone viruses is unclear. We demonstrate that cytotoxic T lymphocyte–deficient TgH(KL25) mice, which are transgenic for the heavy chain of the lymphocytic choriomeningitis virus (LCMV...

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Autores principales: Hangartner, Lars, Zellweger, Raphaël M., Giobbi, Mattia, Weber, Jacqueline, Eschli, Bruno, McCoy, Kathy D., Harris, Nicola, Recher, Mike, Zinkernagel, Rolf M., Hengartner, Hans
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118372/
https://www.ncbi.nlm.nih.gov/pubmed/16880253
http://dx.doi.org/10.1084/jem.20051557
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author Hangartner, Lars
Zellweger, Raphaël M.
Giobbi, Mattia
Weber, Jacqueline
Eschli, Bruno
McCoy, Kathy D.
Harris, Nicola
Recher, Mike
Zinkernagel, Rolf M.
Hengartner, Hans
author_facet Hangartner, Lars
Zellweger, Raphaël M.
Giobbi, Mattia
Weber, Jacqueline
Eschli, Bruno
McCoy, Kathy D.
Harris, Nicola
Recher, Mike
Zinkernagel, Rolf M.
Hengartner, Hans
author_sort Hangartner, Lars
collection PubMed
description The biological relevance of nonneutralizing antibodies elicited early after infection with noncytopathic persistence-prone viruses is unclear. We demonstrate that cytotoxic T lymphocyte–deficient TgH(KL25) mice, which are transgenic for the heavy chain of the lymphocytic choriomeningitis virus (LCMV)–neutralizing monoclonal antibody KL25, mount a focused neutralizing antibody response following LCMV infection, and that this results in the emergence of neutralization escape virus variants. Further investigation revealed that some of the escape variants that arose early after infection could still bind to the selecting antibody. In contrast, no antibody binding could be detected for late isolates, indicating that binding, but nonneutralizing, antibodies exerted a selective pressure on the virus. Infection of naive TgH(KL25) mice with distinct escape viruses differing in their antibody-binding properties revealed that nonneutralizing antibodies accelerated clearance of antibody-binding virus variants in a partly complement-dependent manner. Virus variants that did not bind antibodies were not affected. We therefore conclude that nonneutralizing antibodies binding to the same antigenic site as neutralizing antibodies are biologically relevant by limiting early viral spread.
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spelling pubmed-21183722007-12-13 Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread Hangartner, Lars Zellweger, Raphaël M. Giobbi, Mattia Weber, Jacqueline Eschli, Bruno McCoy, Kathy D. Harris, Nicola Recher, Mike Zinkernagel, Rolf M. Hengartner, Hans J Exp Med Articles The biological relevance of nonneutralizing antibodies elicited early after infection with noncytopathic persistence-prone viruses is unclear. We demonstrate that cytotoxic T lymphocyte–deficient TgH(KL25) mice, which are transgenic for the heavy chain of the lymphocytic choriomeningitis virus (LCMV)–neutralizing monoclonal antibody KL25, mount a focused neutralizing antibody response following LCMV infection, and that this results in the emergence of neutralization escape virus variants. Further investigation revealed that some of the escape variants that arose early after infection could still bind to the selecting antibody. In contrast, no antibody binding could be detected for late isolates, indicating that binding, but nonneutralizing, antibodies exerted a selective pressure on the virus. Infection of naive TgH(KL25) mice with distinct escape viruses differing in their antibody-binding properties revealed that nonneutralizing antibodies accelerated clearance of antibody-binding virus variants in a partly complement-dependent manner. Virus variants that did not bind antibodies were not affected. We therefore conclude that nonneutralizing antibodies binding to the same antigenic site as neutralizing antibodies are biologically relevant by limiting early viral spread. The Rockefeller University Press 2006-08-07 /pmc/articles/PMC2118372/ /pubmed/16880253 http://dx.doi.org/10.1084/jem.20051557 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Hangartner, Lars
Zellweger, Raphaël M.
Giobbi, Mattia
Weber, Jacqueline
Eschli, Bruno
McCoy, Kathy D.
Harris, Nicola
Recher, Mike
Zinkernagel, Rolf M.
Hengartner, Hans
Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread
title Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread
title_full Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread
title_fullStr Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread
title_full_unstemmed Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread
title_short Nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread
title_sort nonneutralizing antibodies binding to the surface glycoprotein of lymphocytic choriomeningitis virus reduce early virus spread
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118372/
https://www.ncbi.nlm.nih.gov/pubmed/16880253
http://dx.doi.org/10.1084/jem.20051557
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