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Functionally specialized junctions between endothelial cells of lymphatic vessels

Recirculation of fluid and cells through lymphatic vessels plays a key role in normal tissue homeostasis, inflammatory diseases, and cancer. Despite recent advances in understanding lymphatic function (Alitalo, K., T. Tammela, and T.V. Petrova. 2005. Nature. 438:946–953), the cellular features respo...

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Detalles Bibliográficos
Autores principales: Baluk, Peter, Fuxe, Jonas, Hashizume, Hiroya, Romano, Talia, Lashnits, Erin, Butz, Stefan, Vestweber, Dietmar, Corada, Monica, Molendini, Cinzia, Dejana, Elisabetta, McDonald, Donald M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118470/
https://www.ncbi.nlm.nih.gov/pubmed/17846148
http://dx.doi.org/10.1084/jem.20062596
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author Baluk, Peter
Fuxe, Jonas
Hashizume, Hiroya
Romano, Talia
Lashnits, Erin
Butz, Stefan
Vestweber, Dietmar
Corada, Monica
Molendini, Cinzia
Dejana, Elisabetta
McDonald, Donald M.
author_facet Baluk, Peter
Fuxe, Jonas
Hashizume, Hiroya
Romano, Talia
Lashnits, Erin
Butz, Stefan
Vestweber, Dietmar
Corada, Monica
Molendini, Cinzia
Dejana, Elisabetta
McDonald, Donald M.
author_sort Baluk, Peter
collection PubMed
description Recirculation of fluid and cells through lymphatic vessels plays a key role in normal tissue homeostasis, inflammatory diseases, and cancer. Despite recent advances in understanding lymphatic function (Alitalo, K., T. Tammela, and T.V. Petrova. 2005. Nature. 438:946–953), the cellular features responsible for entry of fluid and cells into lymphatics are incompletely understood. We report the presence of novel junctions between endothelial cells of initial lymphatics at likely sites of fluid entry. Overlapping flaps at borders of oak leaf–shaped endothelial cells of initial lymphatics lacked junctions at the tip but were anchored on the sides by discontinuous button-like junctions (buttons) that differed from conventional, continuous, zipper-like junctions (zippers) in collecting lymphatics and blood vessels. However, both buttons and zippers were composed of vascular endothelial cadherin (VE-cadherin) and tight junction–associated proteins, including occludin, claudin-5, zonula occludens–1, junctional adhesion molecule–A, and endothelial cell–selective adhesion molecule. In C57BL/6 mice, VE-cadherin was required for maintenance of junctional integrity, but platelet/endothelial cell adhesion molecule–1 was not. Growing tips of lymphatic sprouts had zippers, not buttons, suggesting that buttons are specialized junctions rather than immature ones. Our findings suggest that fluid enters throughout initial lymphatics via openings between buttons, which open and close without disrupting junctional integrity, but most leukocytes enter the proximal half of initial lymphatics.
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spelling pubmed-21184702008-04-01 Functionally specialized junctions between endothelial cells of lymphatic vessels Baluk, Peter Fuxe, Jonas Hashizume, Hiroya Romano, Talia Lashnits, Erin Butz, Stefan Vestweber, Dietmar Corada, Monica Molendini, Cinzia Dejana, Elisabetta McDonald, Donald M. J Exp Med Articles Recirculation of fluid and cells through lymphatic vessels plays a key role in normal tissue homeostasis, inflammatory diseases, and cancer. Despite recent advances in understanding lymphatic function (Alitalo, K., T. Tammela, and T.V. Petrova. 2005. Nature. 438:946–953), the cellular features responsible for entry of fluid and cells into lymphatics are incompletely understood. We report the presence of novel junctions between endothelial cells of initial lymphatics at likely sites of fluid entry. Overlapping flaps at borders of oak leaf–shaped endothelial cells of initial lymphatics lacked junctions at the tip but were anchored on the sides by discontinuous button-like junctions (buttons) that differed from conventional, continuous, zipper-like junctions (zippers) in collecting lymphatics and blood vessels. However, both buttons and zippers were composed of vascular endothelial cadherin (VE-cadherin) and tight junction–associated proteins, including occludin, claudin-5, zonula occludens–1, junctional adhesion molecule–A, and endothelial cell–selective adhesion molecule. In C57BL/6 mice, VE-cadherin was required for maintenance of junctional integrity, but platelet/endothelial cell adhesion molecule–1 was not. Growing tips of lymphatic sprouts had zippers, not buttons, suggesting that buttons are specialized junctions rather than immature ones. Our findings suggest that fluid enters throughout initial lymphatics via openings between buttons, which open and close without disrupting junctional integrity, but most leukocytes enter the proximal half of initial lymphatics. The Rockefeller University Press 2007-10-01 /pmc/articles/PMC2118470/ /pubmed/17846148 http://dx.doi.org/10.1084/jem.20062596 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Baluk, Peter
Fuxe, Jonas
Hashizume, Hiroya
Romano, Talia
Lashnits, Erin
Butz, Stefan
Vestweber, Dietmar
Corada, Monica
Molendini, Cinzia
Dejana, Elisabetta
McDonald, Donald M.
Functionally specialized junctions between endothelial cells of lymphatic vessels
title Functionally specialized junctions between endothelial cells of lymphatic vessels
title_full Functionally specialized junctions between endothelial cells of lymphatic vessels
title_fullStr Functionally specialized junctions between endothelial cells of lymphatic vessels
title_full_unstemmed Functionally specialized junctions between endothelial cells of lymphatic vessels
title_short Functionally specialized junctions between endothelial cells of lymphatic vessels
title_sort functionally specialized junctions between endothelial cells of lymphatic vessels
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118470/
https://www.ncbi.nlm.nih.gov/pubmed/17846148
http://dx.doi.org/10.1084/jem.20062596
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