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Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines

The minimal region of the human tumor necrosis factor alpha (TNF-alpha) gene promoter necessary for its transcriptional induction by phorbol esters (PMA) in human T and B lymphocyte cell lines has been localized between -52 and +89 nucleotides (nt) relative to the gene's transcriptional start s...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1991
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118869/
https://www.ncbi.nlm.nih.gov/pubmed/2056282
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collection PubMed
description The minimal region of the human tumor necrosis factor alpha (TNF-alpha) gene promoter necessary for its transcriptional induction by phorbol esters (PMA) in human T and B lymphocyte cell lines has been localized between -52 and +89 nucleotides (nt) relative to the gene's transcriptional start site. Comparison of these sequences to those required to mediate virus or lipopolysaccharide (LPS) induction of the gene reveal significant differences, and thus, the sequence requirements for PMA induction are distinct from those that mediate induction by virus or LPS. Although three sites in the TNF-alpha promoter (kappa 1, kappa 2, and kappa 3) specifically bind the transcription factor NF-kappa B in lymphoid nuclear extracts, TNF-alpha mRNA induction by PMA does not correlate with NF-kappa B binding activities displayed by different T and B cell lines. Moreover, kappa 1- kappa 3 can each be deleted from the TNF-alpha promoter with little effect on the gene's inducibility by PMA. Therefore, TNF-alpha mRNA induction by PMA, like its induction by virus and LPS, is not primarily mediated by NF-kappa B, but rather is mediated through other sequences and protein factors. Surprisingly, multimers of kappa 1-kappa 3 can confer PMA inducibility on a heterologous promoter in a B (Raji), but not a T (HUT78) cell line. However they are not functional on a truncated TNF-alpha promoter, indicating that promoter context and cell type specificity influence the PMA inducible function of these NF-kappa B binding sites.
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spelling pubmed-21188692008-04-17 Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines J Exp Med Articles The minimal region of the human tumor necrosis factor alpha (TNF-alpha) gene promoter necessary for its transcriptional induction by phorbol esters (PMA) in human T and B lymphocyte cell lines has been localized between -52 and +89 nucleotides (nt) relative to the gene's transcriptional start site. Comparison of these sequences to those required to mediate virus or lipopolysaccharide (LPS) induction of the gene reveal significant differences, and thus, the sequence requirements for PMA induction are distinct from those that mediate induction by virus or LPS. Although three sites in the TNF-alpha promoter (kappa 1, kappa 2, and kappa 3) specifically bind the transcription factor NF-kappa B in lymphoid nuclear extracts, TNF-alpha mRNA induction by PMA does not correlate with NF-kappa B binding activities displayed by different T and B cell lines. Moreover, kappa 1- kappa 3 can each be deleted from the TNF-alpha promoter with little effect on the gene's inducibility by PMA. Therefore, TNF-alpha mRNA induction by PMA, like its induction by virus and LPS, is not primarily mediated by NF-kappa B, but rather is mediated through other sequences and protein factors. Surprisingly, multimers of kappa 1-kappa 3 can confer PMA inducibility on a heterologous promoter in a B (Raji), but not a T (HUT78) cell line. However they are not functional on a truncated TNF-alpha promoter, indicating that promoter context and cell type specificity influence the PMA inducible function of these NF-kappa B binding sites. The Rockefeller University Press 1991-07-01 /pmc/articles/PMC2118869/ /pubmed/2056282 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines
title Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines
title_full Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines
title_fullStr Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines
title_full_unstemmed Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines
title_short Human tumor necrosis factor alpha gene regulation in phorbol ester stimulated T and B cell lines
title_sort human tumor necrosis factor alpha gene regulation in phorbol ester stimulated t and b cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118869/
https://www.ncbi.nlm.nih.gov/pubmed/2056282