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The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis
Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically medi...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1991
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118921/ https://www.ncbi.nlm.nih.gov/pubmed/1856625 |
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collection | PubMed |
description | Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically mediated by iron; parasites cultured in a low- iron medium in the presence of salts other than iron were unresponsive to changes in adherence levels. Expression of the higher adherence property, by parasites grown first in low-iron medium followed by supplementation with iron, was a function of time, and the extent of cytoadherence was proportional to the concentration of iron added to the medium. Lactoferrin, an important iron source for trichomonads at the site of infection, elevated adherence of the parasite to epithelial cells, demonstrating the likely in vivo modulation of adherence by iron. The alteration of levels of adherence caused by iron was determined to be a reflection of gene expression of previously characterized trichomonad adhesins. Parasites grown under iron-replete conditions had higher quantities of surface-exposed adhesins, and this was a result of increased synthesis of adhesins. Actinomycin D and alpha-amanitin prevented expression of adhesin molecules, which resulted in decreased cytoadherence, showing that adhesin synthesis was dependent on gene transcription. Data indicated that genes encoding the four trichomonad adhesins are coordinately regulated by iron. |
format | Text |
id | pubmed-2118921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1991 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21189212008-04-17 The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis J Exp Med Articles Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically mediated by iron; parasites cultured in a low- iron medium in the presence of salts other than iron were unresponsive to changes in adherence levels. Expression of the higher adherence property, by parasites grown first in low-iron medium followed by supplementation with iron, was a function of time, and the extent of cytoadherence was proportional to the concentration of iron added to the medium. Lactoferrin, an important iron source for trichomonads at the site of infection, elevated adherence of the parasite to epithelial cells, demonstrating the likely in vivo modulation of adherence by iron. The alteration of levels of adherence caused by iron was determined to be a reflection of gene expression of previously characterized trichomonad adhesins. Parasites grown under iron-replete conditions had higher quantities of surface-exposed adhesins, and this was a result of increased synthesis of adhesins. Actinomycin D and alpha-amanitin prevented expression of adhesin molecules, which resulted in decreased cytoadherence, showing that adhesin synthesis was dependent on gene transcription. Data indicated that genes encoding the four trichomonad adhesins are coordinately regulated by iron. The Rockefeller University Press 1991-08-01 /pmc/articles/PMC2118921/ /pubmed/1856625 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis |
title | The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis |
title_full | The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis |
title_fullStr | The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis |
title_full_unstemmed | The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis |
title_short | The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis |
title_sort | regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan trichomonas vaginalis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118921/ https://www.ncbi.nlm.nih.gov/pubmed/1856625 |