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The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis

Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically medi...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1991
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118921/
https://www.ncbi.nlm.nih.gov/pubmed/1856625
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description Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically mediated by iron; parasites cultured in a low- iron medium in the presence of salts other than iron were unresponsive to changes in adherence levels. Expression of the higher adherence property, by parasites grown first in low-iron medium followed by supplementation with iron, was a function of time, and the extent of cytoadherence was proportional to the concentration of iron added to the medium. Lactoferrin, an important iron source for trichomonads at the site of infection, elevated adherence of the parasite to epithelial cells, demonstrating the likely in vivo modulation of adherence by iron. The alteration of levels of adherence caused by iron was determined to be a reflection of gene expression of previously characterized trichomonad adhesins. Parasites grown under iron-replete conditions had higher quantities of surface-exposed adhesins, and this was a result of increased synthesis of adhesins. Actinomycin D and alpha-amanitin prevented expression of adhesin molecules, which resulted in decreased cytoadherence, showing that adhesin synthesis was dependent on gene transcription. Data indicated that genes encoding the four trichomonad adhesins are coordinately regulated by iron.
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spelling pubmed-21189212008-04-17 The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis J Exp Med Articles Levels of adherence of Trichomonas vaginalis to epithelial cells was found to be modulated by iron. Cytoadherence values were greater than or equal to twofold higher for trichomonads grown in a complex cultivation medium supplemented with iron. This increase in adherence levels was specifically mediated by iron; parasites cultured in a low- iron medium in the presence of salts other than iron were unresponsive to changes in adherence levels. Expression of the higher adherence property, by parasites grown first in low-iron medium followed by supplementation with iron, was a function of time, and the extent of cytoadherence was proportional to the concentration of iron added to the medium. Lactoferrin, an important iron source for trichomonads at the site of infection, elevated adherence of the parasite to epithelial cells, demonstrating the likely in vivo modulation of adherence by iron. The alteration of levels of adherence caused by iron was determined to be a reflection of gene expression of previously characterized trichomonad adhesins. Parasites grown under iron-replete conditions had higher quantities of surface-exposed adhesins, and this was a result of increased synthesis of adhesins. Actinomycin D and alpha-amanitin prevented expression of adhesin molecules, which resulted in decreased cytoadherence, showing that adhesin synthesis was dependent on gene transcription. Data indicated that genes encoding the four trichomonad adhesins are coordinately regulated by iron. The Rockefeller University Press 1991-08-01 /pmc/articles/PMC2118921/ /pubmed/1856625 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis
title The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis
title_full The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis
title_fullStr The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis
title_full_unstemmed The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis
title_short The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis
title_sort regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan trichomonas vaginalis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118921/
https://www.ncbi.nlm.nih.gov/pubmed/1856625