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Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice

A cDNA library of TCR beta chain transcripts from BALB/c thymocytes was constructed using anchored polymerase chain reaction (PCR). Screening of this library led to the identification of a V beta gene segment, V beta 20, structurally related to V beta 3 and V beta 17. Genomic analysis of mice displa...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1991
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118986/
https://www.ncbi.nlm.nih.gov/pubmed/1834762
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description A cDNA library of TCR beta chain transcripts from BALB/c thymocytes was constructed using anchored polymerase chain reaction (PCR). Screening of this library led to the identification of a V beta gene segment, V beta 20, structurally related to V beta 3 and V beta 17. Genomic analysis of mice displaying deletions in their V beta loci, together with mapping of cosmid clones, situated V beta 20 2.5 kb beside V beta 17. The expression of V beta 20 was estimated by PCR in mice of different H-2 and Mls types. Peripheral T cells from H-2k and H-2d mice did not express V beta 20, whereas in I-E-negative mice (C57Bl/6 and SJL), V beta 20 transcripts were detected. The lack of V beta 20 transcripts in (C57Bl/6 x CBA/J)F1, (C57Bl/6 x BALB/c)F1, and in congenic B6.H-2k mice suggests that the differential use of V beta 20 is due to an I-E-mediated clonal deletion process. The involvement of the Mls super antigens was excluded by analysis of all Mls type combinations. The nature of the V beta 20-deleting element(s) is discussed in the context of the I-E/superantigen systems controlling the expression of V beta 11 and V beta 17.
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spelling pubmed-21189862008-04-17 Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice J Exp Med Articles A cDNA library of TCR beta chain transcripts from BALB/c thymocytes was constructed using anchored polymerase chain reaction (PCR). Screening of this library led to the identification of a V beta gene segment, V beta 20, structurally related to V beta 3 and V beta 17. Genomic analysis of mice displaying deletions in their V beta loci, together with mapping of cosmid clones, situated V beta 20 2.5 kb beside V beta 17. The expression of V beta 20 was estimated by PCR in mice of different H-2 and Mls types. Peripheral T cells from H-2k and H-2d mice did not express V beta 20, whereas in I-E-negative mice (C57Bl/6 and SJL), V beta 20 transcripts were detected. The lack of V beta 20 transcripts in (C57Bl/6 x CBA/J)F1, (C57Bl/6 x BALB/c)F1, and in congenic B6.H-2k mice suggests that the differential use of V beta 20 is due to an I-E-mediated clonal deletion process. The involvement of the Mls super antigens was excluded by analysis of all Mls type combinations. The nature of the V beta 20-deleting element(s) is discussed in the context of the I-E/superantigen systems controlling the expression of V beta 11 and V beta 17. The Rockefeller University Press 1991-11-01 /pmc/articles/PMC2118986/ /pubmed/1834762 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice
title Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice
title_full Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice
title_fullStr Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice
title_full_unstemmed Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice
title_short Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice
title_sort identification of a t cell receptor beta chain variable region, v beta 20, that is differentially expressed in various strains of mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2118986/
https://www.ncbi.nlm.nih.gov/pubmed/1834762