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Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]
Decay-accelerating factor (DAF) is a 70-kD membrane glycoprotein that prevents complement (C)-mediated hemolysis by blocking the assembly or accelerating the decay of C3 convertase. Purified DAF is known to incorporate into the membrane of DAF-deficient cells, inhibiting lysis. Since Schistosoma man...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1991
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119036/ https://www.ncbi.nlm.nih.gov/pubmed/1720809 |
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collection | PubMed |
description | Decay-accelerating factor (DAF) is a 70-kD membrane glycoprotein that prevents complement (C)-mediated hemolysis by blocking the assembly or accelerating the decay of C3 convertase. Purified DAF is known to incorporate into the membrane of DAF-deficient cells, inhibiting lysis. Since Schistosoma mansoni is a blood-dwelling parasite, we investigated whether DAF can be transferred from human erythrocytes to the worm and protect it against C-mediated killing in vitro. We have found that schistosomula (schla) incubated with normal human erythrocytes (N-HuE), but not with DAF-deficient erythrocytes, become resistant to C damage in vitro. Protected parasites acquire a 70-kD surface protein which can be immunoprecipitated by anti-DAF antibodies. The acquired resistance is abrogated by treatment of N-HuE-incubated parasites with anti-DAF antibody. These results indicate that, in vitro, N-HuE DAF can be transferred to schla, and suggest its participation in preventing their C-mediated killing. This could represent an important strategy of parasites to evade the host's immune response in vivo. |
format | Text |
id | pubmed-2119036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1991 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21190362008-04-17 Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] J Exp Med Articles Decay-accelerating factor (DAF) is a 70-kD membrane glycoprotein that prevents complement (C)-mediated hemolysis by blocking the assembly or accelerating the decay of C3 convertase. Purified DAF is known to incorporate into the membrane of DAF-deficient cells, inhibiting lysis. Since Schistosoma mansoni is a blood-dwelling parasite, we investigated whether DAF can be transferred from human erythrocytes to the worm and protect it against C-mediated killing in vitro. We have found that schistosomula (schla) incubated with normal human erythrocytes (N-HuE), but not with DAF-deficient erythrocytes, become resistant to C damage in vitro. Protected parasites acquire a 70-kD surface protein which can be immunoprecipitated by anti-DAF antibodies. The acquired resistance is abrogated by treatment of N-HuE-incubated parasites with anti-DAF antibody. These results indicate that, in vitro, N-HuE DAF can be transferred to schla, and suggest its participation in preventing their C-mediated killing. This could represent an important strategy of parasites to evade the host's immune response in vivo. The Rockefeller University Press 1991-12-01 /pmc/articles/PMC2119036/ /pubmed/1720809 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] |
title | Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] |
title_full | Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] |
title_fullStr | Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] |
title_full_unstemmed | Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] |
title_short | Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] |
title_sort | role of human decay-accelerating factor in the evasion of schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in j exp med 1992 feb 1;175(2):619] |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119036/ https://www.ncbi.nlm.nih.gov/pubmed/1720809 |