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Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]

Decay-accelerating factor (DAF) is a 70-kD membrane glycoprotein that prevents complement (C)-mediated hemolysis by blocking the assembly or accelerating the decay of C3 convertase. Purified DAF is known to incorporate into the membrane of DAF-deficient cells, inhibiting lysis. Since Schistosoma man...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1991
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119036/
https://www.ncbi.nlm.nih.gov/pubmed/1720809
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collection PubMed
description Decay-accelerating factor (DAF) is a 70-kD membrane glycoprotein that prevents complement (C)-mediated hemolysis by blocking the assembly or accelerating the decay of C3 convertase. Purified DAF is known to incorporate into the membrane of DAF-deficient cells, inhibiting lysis. Since Schistosoma mansoni is a blood-dwelling parasite, we investigated whether DAF can be transferred from human erythrocytes to the worm and protect it against C-mediated killing in vitro. We have found that schistosomula (schla) incubated with normal human erythrocytes (N-HuE), but not with DAF-deficient erythrocytes, become resistant to C damage in vitro. Protected parasites acquire a 70-kD surface protein which can be immunoprecipitated by anti-DAF antibodies. The acquired resistance is abrogated by treatment of N-HuE-incubated parasites with anti-DAF antibody. These results indicate that, in vitro, N-HuE DAF can be transferred to schla, and suggest its participation in preventing their C-mediated killing. This could represent an important strategy of parasites to evade the host's immune response in vivo.
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spelling pubmed-21190362008-04-17 Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619] J Exp Med Articles Decay-accelerating factor (DAF) is a 70-kD membrane glycoprotein that prevents complement (C)-mediated hemolysis by blocking the assembly or accelerating the decay of C3 convertase. Purified DAF is known to incorporate into the membrane of DAF-deficient cells, inhibiting lysis. Since Schistosoma mansoni is a blood-dwelling parasite, we investigated whether DAF can be transferred from human erythrocytes to the worm and protect it against C-mediated killing in vitro. We have found that schistosomula (schla) incubated with normal human erythrocytes (N-HuE), but not with DAF-deficient erythrocytes, become resistant to C damage in vitro. Protected parasites acquire a 70-kD surface protein which can be immunoprecipitated by anti-DAF antibodies. The acquired resistance is abrogated by treatment of N-HuE-incubated parasites with anti-DAF antibody. These results indicate that, in vitro, N-HuE DAF can be transferred to schla, and suggest its participation in preventing their C-mediated killing. This could represent an important strategy of parasites to evade the host's immune response in vivo. The Rockefeller University Press 1991-12-01 /pmc/articles/PMC2119036/ /pubmed/1720809 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]
title Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]
title_full Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]
title_fullStr Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]
title_full_unstemmed Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]
title_short Role of human decay-accelerating factor in the evasion of Schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in J Exp Med 1992 Feb 1;175(2):619]
title_sort role of human decay-accelerating factor in the evasion of schistosoma mansoni from the complement-mediated killing in vitro [published erratum appears in j exp med 1992 feb 1;175(2):619]
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119036/
https://www.ncbi.nlm.nih.gov/pubmed/1720809