Mapping of the nu gene using congenic nude strains and in situ hybridization

The chromosomal location of the nu gene, which is responsible for hairlessness and athymus, was determined using six DNA markers (interleukin 3 [Il-3], Myhs, Acrb, Evi-2, Mpo, and Hox-2) on mouse chromosome 11. We constructed the high-resolution physical mapping of the six DNA markers on chromosome...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1992
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119153/
https://www.ncbi.nlm.nih.gov/pubmed/1346798
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collection PubMed
description The chromosomal location of the nu gene, which is responsible for hairlessness and athymus, was determined using six DNA markers (interleukin 3 [Il-3], Myhs, Acrb, Evi-2, Mpo, and Hox-2) on mouse chromosome 11. We constructed the high-resolution physical mapping of the six DNA markers on chromosome 11 by in situ hybridization using fluorescence-labeled cosmid probes. The results indicate the order of centromere-(41cM)-Il-3-(3cM)-Myhs- (4cM)-Acrb-(6cM)-Evi-2-(3cM)-Mpo- (5cM)- Hox-2. We have used congenic nude strains and examined which of the six DNA markers were derived from the original nude mouse. We found the Evi-2 locus is linked to the nu gene in all the informative, independent congenic nude strains. From these data, we could estimate the location of the nu gene, not only genetically but also physically within a region that spans approximately 17 megabases (9 cM) between the Acrb and Mpo genes.
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spelling pubmed-21191532008-04-16 Mapping of the nu gene using congenic nude strains and in situ hybridization J Exp Med Articles The chromosomal location of the nu gene, which is responsible for hairlessness and athymus, was determined using six DNA markers (interleukin 3 [Il-3], Myhs, Acrb, Evi-2, Mpo, and Hox-2) on mouse chromosome 11. We constructed the high-resolution physical mapping of the six DNA markers on chromosome 11 by in situ hybridization using fluorescence-labeled cosmid probes. The results indicate the order of centromere-(41cM)-Il-3-(3cM)-Myhs- (4cM)-Acrb-(6cM)-Evi-2-(3cM)-Mpo- (5cM)- Hox-2. We have used congenic nude strains and examined which of the six DNA markers were derived from the original nude mouse. We found the Evi-2 locus is linked to the nu gene in all the informative, independent congenic nude strains. From these data, we could estimate the location of the nu gene, not only genetically but also physically within a region that spans approximately 17 megabases (9 cM) between the Acrb and Mpo genes. The Rockefeller University Press 1992-03-01 /pmc/articles/PMC2119153/ /pubmed/1346798 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Mapping of the nu gene using congenic nude strains and in situ hybridization
title Mapping of the nu gene using congenic nude strains and in situ hybridization
title_full Mapping of the nu gene using congenic nude strains and in situ hybridization
title_fullStr Mapping of the nu gene using congenic nude strains and in situ hybridization
title_full_unstemmed Mapping of the nu gene using congenic nude strains and in situ hybridization
title_short Mapping of the nu gene using congenic nude strains and in situ hybridization
title_sort mapping of the nu gene using congenic nude strains and in situ hybridization
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119153/
https://www.ncbi.nlm.nih.gov/pubmed/1346798

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