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A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis
The minimum structural requirements for peptide interactions with major histocompatibility complex (MHC) class II molecules and with T cell receptors (TCRs) were examined. In this report we show that substituting alanines at all but five amino acids in the myelin basic protein (MBP) peptide Ac1-11 d...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1992
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119337/ https://www.ncbi.nlm.nih.gov/pubmed/1380066 |
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collection | PubMed |
description | The minimum structural requirements for peptide interactions with major histocompatibility complex (MHC) class II molecules and with T cell receptors (TCRs) were examined. In this report we show that substituting alanines at all but five amino acids in the myelin basic protein (MBP) peptide Ac1-11 does not alter its ability to bind A alpha uA beta u (MHC class II molecules), to stimulate specific T cells and, surprisingly, to induce experimental autoimmune encephalomyelitis (EAE) in (PL/J x SJL/J)F1 mice. Most other amino acid side chains in the Ac1- 11 peptide are essentially irrelevant for T cell stimulation and for disease induction. Further analysis revealed that binding to A alpha uA beta u occurred with a peptide that consists mainly of alanines and only three of the original residues of Ac1-11. Moreover, when used as a coimmunogen with MBP Ac1-11, this peptide inhibited EAE. The finding that a specific in vivo response can be generated by a peptide containing only five native residues provides evidence that disease- inducing TCRs recognize only a very short sequence of the MHC-bound peptide. |
format | Text |
id | pubmed-2119337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21193372008-04-16 A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis J Exp Med Articles The minimum structural requirements for peptide interactions with major histocompatibility complex (MHC) class II molecules and with T cell receptors (TCRs) were examined. In this report we show that substituting alanines at all but five amino acids in the myelin basic protein (MBP) peptide Ac1-11 does not alter its ability to bind A alpha uA beta u (MHC class II molecules), to stimulate specific T cells and, surprisingly, to induce experimental autoimmune encephalomyelitis (EAE) in (PL/J x SJL/J)F1 mice. Most other amino acid side chains in the Ac1- 11 peptide are essentially irrelevant for T cell stimulation and for disease induction. Further analysis revealed that binding to A alpha uA beta u occurred with a peptide that consists mainly of alanines and only three of the original residues of Ac1-11. Moreover, when used as a coimmunogen with MBP Ac1-11, this peptide inhibited EAE. The finding that a specific in vivo response can be generated by a peptide containing only five native residues provides evidence that disease- inducing TCRs recognize only a very short sequence of the MHC-bound peptide. The Rockefeller University Press 1992-08-01 /pmc/articles/PMC2119337/ /pubmed/1380066 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis |
title | A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis |
title_full | A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis |
title_fullStr | A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis |
title_full_unstemmed | A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis |
title_short | A polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis |
title_sort | polyalanine peptide with only five native myelin basic protein residues induces autoimmune encephalomyelitis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119337/ https://www.ncbi.nlm.nih.gov/pubmed/1380066 |