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Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells
Terminally differentiated plasma cells and mouse T cells do not express major histocompatibility complex (MHC) class II genes although class II gene expression is observed in pre-B and mature B cells as well as in activated human T cells. Transient heterokaryons were prepared and analyzed to investi...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1992
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119411/ https://www.ncbi.nlm.nih.gov/pubmed/1402690 |
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collection | PubMed |
description | Terminally differentiated plasma cells and mouse T cells do not express major histocompatibility complex (MHC) class II genes although class II gene expression is observed in pre-B and mature B cells as well as in activated human T cells. Transient heterokaryons were prepared and analyzed to investigate the mechanisms of inactivation of MHC class II gene in mouse plasmacytoma cells and mouse T cells. The endogenous MHC class II genes in both mouse plasmacytoma cells and mouse T cells can be reactivated by factors present in B cells. This reactivation of class II gene is also observed by fusion with a human T cell line which expresses MHC class II genes, but not with a class II negative human T cell line. It appears that the loss of MHC class II gene expression during the terminal differentiation of B cells or T cell lineage is due to absence of positive regulatory factor(s) necessary for class II transcription. |
format | Text |
id | pubmed-2119411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21194112008-04-16 Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells J Exp Med Articles Terminally differentiated plasma cells and mouse T cells do not express major histocompatibility complex (MHC) class II genes although class II gene expression is observed in pre-B and mature B cells as well as in activated human T cells. Transient heterokaryons were prepared and analyzed to investigate the mechanisms of inactivation of MHC class II gene in mouse plasmacytoma cells and mouse T cells. The endogenous MHC class II genes in both mouse plasmacytoma cells and mouse T cells can be reactivated by factors present in B cells. This reactivation of class II gene is also observed by fusion with a human T cell line which expresses MHC class II genes, but not with a class II negative human T cell line. It appears that the loss of MHC class II gene expression during the terminal differentiation of B cells or T cell lineage is due to absence of positive regulatory factor(s) necessary for class II transcription. The Rockefeller University Press 1992-11-01 /pmc/articles/PMC2119411/ /pubmed/1402690 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells |
title | Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells |
title_full | Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells |
title_fullStr | Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells |
title_full_unstemmed | Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells |
title_short | Reactivation of a major histocompatibility complex class II gene in mouse plasmacytoma cells and mouse T cells |
title_sort | reactivation of a major histocompatibility complex class ii gene in mouse plasmacytoma cells and mouse t cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119411/ https://www.ncbi.nlm.nih.gov/pubmed/1402690 |