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Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells
In an attempt to characterize genes participating in the allergic late phase reaction, we have isolated a novel intercrine/chemokine (called MARC) from a cDNA library of the stimulated mouse mast cell line, CPII. As measured by Northern blotting, it is strongly upregulated at the mRNA level after th...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1992
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119461/ https://www.ncbi.nlm.nih.gov/pubmed/1281219 |
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collection | PubMed |
description | In an attempt to characterize genes participating in the allergic late phase reaction, we have isolated a novel intercrine/chemokine (called MARC) from a cDNA library of the stimulated mouse mast cell line, CPII. As measured by Northern blotting, it is strongly upregulated at the mRNA level after the physiological challenge of the cells with immunoglobulin (Ig)E plus antigen. Unstimulated cells completely lack significant, stable expression, as do a number of other, different cell lines (uninduced and induced) and mouse tissues. In contrast to the Northern blot analysis, a polymerase chain reaction (PCR) analysis, performed on CPII cells and on Percoll gradient purified mouse peritoneal mast cells, revealed a basal level of transcription in the uninduced stage. After 2 h of IgE plus antigen challenge, a quantitative reverse transcriptase-PCR, using a spiked in MIMIC, showed a level of transcripts more than 100-fold higher in the CPII cells and 5-20-fold higher in purified mouse peritoneal cavity mast cells. This rapid induction after the Fc epsilon RI challenge, the identification of the gene as a member of the chemokine family, and its upregulated expression in peritoneal mast cells, all suggest an involvement in certain acute and chronic pathological mast cell-driven diseases. |
format | Text |
id | pubmed-2119461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21194612008-04-16 Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells J Exp Med Articles In an attempt to characterize genes participating in the allergic late phase reaction, we have isolated a novel intercrine/chemokine (called MARC) from a cDNA library of the stimulated mouse mast cell line, CPII. As measured by Northern blotting, it is strongly upregulated at the mRNA level after the physiological challenge of the cells with immunoglobulin (Ig)E plus antigen. Unstimulated cells completely lack significant, stable expression, as do a number of other, different cell lines (uninduced and induced) and mouse tissues. In contrast to the Northern blot analysis, a polymerase chain reaction (PCR) analysis, performed on CPII cells and on Percoll gradient purified mouse peritoneal mast cells, revealed a basal level of transcription in the uninduced stage. After 2 h of IgE plus antigen challenge, a quantitative reverse transcriptase-PCR, using a spiked in MIMIC, showed a level of transcripts more than 100-fold higher in the CPII cells and 5-20-fold higher in purified mouse peritoneal cavity mast cells. This rapid induction after the Fc epsilon RI challenge, the identification of the gene as a member of the chemokine family, and its upregulated expression in peritoneal mast cells, all suggest an involvement in certain acute and chronic pathological mast cell-driven diseases. The Rockefeller University Press 1992-12-01 /pmc/articles/PMC2119461/ /pubmed/1281219 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells |
title | Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells |
title_full | Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells |
title_fullStr | Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells |
title_full_unstemmed | Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells |
title_short | Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells |
title_sort | immunoglobulin e plus antigen challenge induces a novel intercrine/chemokine in mouse mast cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119461/ https://www.ncbi.nlm.nih.gov/pubmed/1281219 |