Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes

The 43-kD protein is a peripheral membrane protein that is in approximately 1:1 stoichiometry with the acetylcholine receptor (AChR) in vertebrate muscle cells and colocalizes with it in the postsynaptic membrane. To investigate the role of the 43-kD protein in AChR clustering, we have isolated C2 m...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1993
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119616/
https://www.ncbi.nlm.nih.gov/pubmed/7686162
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collection PubMed
description The 43-kD protein is a peripheral membrane protein that is in approximately 1:1 stoichiometry with the acetylcholine receptor (AChR) in vertebrate muscle cells and colocalizes with it in the postsynaptic membrane. To investigate the role of the 43-kD protein in AChR clustering, we have isolated C2 muscle cell lines in which some cells overexpress the 43-kD protein. We find that myotubes with increased levels of the 43-kD protein have small AChR clusters and that those with the highest levels of expression have a drastically reduced number of clusters. Our results suggest that the 1:1 stoichiometry of AChR and 43-kD protein found in muscle cells is important for AChR cluster formation.
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spelling pubmed-21196162008-05-01 Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes J Cell Biol Articles The 43-kD protein is a peripheral membrane protein that is in approximately 1:1 stoichiometry with the acetylcholine receptor (AChR) in vertebrate muscle cells and colocalizes with it in the postsynaptic membrane. To investigate the role of the 43-kD protein in AChR clustering, we have isolated C2 muscle cell lines in which some cells overexpress the 43-kD protein. We find that myotubes with increased levels of the 43-kD protein have small AChR clusters and that those with the highest levels of expression have a drastically reduced number of clusters. Our results suggest that the 1:1 stoichiometry of AChR and 43-kD protein found in muscle cells is important for AChR cluster formation. The Rockefeller University Press 1993-07-01 /pmc/articles/PMC2119616/ /pubmed/7686162 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes
title Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes
title_full Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes
title_fullStr Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes
title_full_unstemmed Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes
title_short Increased expression of the 43-kD protein disrupts acetylcholine receptor clustering in myotubes
title_sort increased expression of the 43-kd protein disrupts acetylcholine receptor clustering in myotubes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119616/
https://www.ncbi.nlm.nih.gov/pubmed/7686162

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