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Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus
The mitogen-activated protein (MAP) kinase signal transduction pathway represents an important mechanism by which growth factors regulate cell function. Targets of the MAP kinase pathway are located within several cellular compartments. Signal transduction therefore requires the localization of MAP...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1993
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119622/ https://www.ncbi.nlm.nih.gov/pubmed/8394846 |
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collection | PubMed |
description | The mitogen-activated protein (MAP) kinase signal transduction pathway represents an important mechanism by which growth factors regulate cell function. Targets of the MAP kinase pathway are located within several cellular compartments. Signal transduction therefore requires the localization of MAP kinase in each sub-cellular compartment that contains physiologically relevant substrates. Here, we show that serum treatment causes the translocation of two human MAP kinase isoforms, p40mapk and p41mapk, from the cytosol into the nucleus. In addition, we report that p41mapk (but not p40mapk) is localized at the cell surface ruffling membrane in serum-treated cells. To investigate whether the protein kinase activity of MAP kinase is required for serum-induced redistribution within the cell, we constructed mutated kinase-negative forms of p40mapk and p41mapk. The kinase-negative MAP kinases were not observed to localize to the cell surface ruffling membrane. In contrast, the kinase-negative MAP kinases were observed to be translocated to the nucleus. Intrinsic MAP kinase activity is therefore required only for localization at the cell surface and is not required for transport into the nucleus. Together, these data demonstrate that the pattern of serum-induced redistribution of p40mapk is different from p41mapk. Thus, in addition to common targets of signal transduction, it is possible that these MAP kinase isoforms may differentially regulate targets located in distinct sub-cellular compartments. |
format | Text |
id | pubmed-2119622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21196222008-05-01 Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus J Cell Biol Articles The mitogen-activated protein (MAP) kinase signal transduction pathway represents an important mechanism by which growth factors regulate cell function. Targets of the MAP kinase pathway are located within several cellular compartments. Signal transduction therefore requires the localization of MAP kinase in each sub-cellular compartment that contains physiologically relevant substrates. Here, we show that serum treatment causes the translocation of two human MAP kinase isoforms, p40mapk and p41mapk, from the cytosol into the nucleus. In addition, we report that p41mapk (but not p40mapk) is localized at the cell surface ruffling membrane in serum-treated cells. To investigate whether the protein kinase activity of MAP kinase is required for serum-induced redistribution within the cell, we constructed mutated kinase-negative forms of p40mapk and p41mapk. The kinase-negative MAP kinases were not observed to localize to the cell surface ruffling membrane. In contrast, the kinase-negative MAP kinases were observed to be translocated to the nucleus. Intrinsic MAP kinase activity is therefore required only for localization at the cell surface and is not required for transport into the nucleus. Together, these data demonstrate that the pattern of serum-induced redistribution of p40mapk is different from p41mapk. Thus, in addition to common targets of signal transduction, it is possible that these MAP kinase isoforms may differentially regulate targets located in distinct sub-cellular compartments. The Rockefeller University Press 1993-09-01 /pmc/articles/PMC2119622/ /pubmed/8394846 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus |
title | Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus |
title_full | Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus |
title_fullStr | Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus |
title_full_unstemmed | Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus |
title_short | Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus |
title_sort | serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119622/ https://www.ncbi.nlm.nih.gov/pubmed/8394846 |