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Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion
We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. Gorlich, M. Wiedmann,...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1993
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119797/ https://www.ncbi.nlm.nih.gov/pubmed/8491769 |
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collection | PubMed |
description | We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. Gorlich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (Gorlich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that Sec61p is a core component of these sites. |
format | Text |
id | pubmed-2119797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21197972008-05-01 Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion J Cell Biol Articles We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. Gorlich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (Gorlich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that Sec61p is a core component of these sites. The Rockefeller University Press 1993-05-02 /pmc/articles/PMC2119797/ /pubmed/8491769 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion |
title | Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion |
title_full | Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion |
title_fullStr | Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion |
title_full_unstemmed | Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion |
title_short | Sec61p is adjacent to nascent type I and type II signal-anchor proteins during their membrane insertion |
title_sort | sec61p is adjacent to nascent type i and type ii signal-anchor proteins during their membrane insertion |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119797/ https://www.ncbi.nlm.nih.gov/pubmed/8491769 |