En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589]

The cDNA encoding epsilon-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP- coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant Hi...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1994
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119964/
https://www.ncbi.nlm.nih.gov/pubmed/8132710
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collection PubMed
description The cDNA encoding epsilon-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP- coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6- tagged epsilon-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.
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spelling pubmed-21199642008-05-01 En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589] J Cell Biol Articles The cDNA encoding epsilon-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP- coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6- tagged epsilon-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit. The Rockefeller University Press 1994-03-02 /pmc/articles/PMC2119964/ /pubmed/8132710 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589]
title En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589]
title_full En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589]
title_fullStr En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589]
title_full_unstemmed En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589]
title_short En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles [published erratum appears in J Cell Biol 1994 Jul;126(2):589]
title_sort en bloc incorporation of coatomer subunits during the assembly of cop- coated vesicles [published erratum appears in j cell biol 1994 jul;126(2):589]
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119964/
https://www.ncbi.nlm.nih.gov/pubmed/8132710

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