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Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway

Karyogamy is the process where haploid nuclei fuse to form a diploid nucleus during yeast mating. We devised a novel genetic screen that identified five new karyogamy (KAR) genes and three new cell fusion (FUS) genes. The kar mutants fell into two classes that represent distinct events in the yeast...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120128/
https://www.ncbi.nlm.nih.gov/pubmed/8051211
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description Karyogamy is the process where haploid nuclei fuse to form a diploid nucleus during yeast mating. We devised a novel genetic screen that identified five new karyogamy (KAR) genes and three new cell fusion (FUS) genes. The kar mutants fell into two classes that represent distinct events in the yeast karyogamy pathway. Class I mutations blocked congression of the nuclei due to cytoplasmic microtubule defects. In Class II mutants, nuclear congression proceeded and the membranes of apposed nuclei were closely aligned but unfused. In vitro, Class II mutant membranes were defective in a homotypic ER/nuclear membrane fusion assay. We propose that Class II mutants define components of a novel membrane fusion complex which functions during vegetative growth and is recruited for karyogamy.
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spelling pubmed-21201282008-05-01 Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway J Cell Biol Articles Karyogamy is the process where haploid nuclei fuse to form a diploid nucleus during yeast mating. We devised a novel genetic screen that identified five new karyogamy (KAR) genes and three new cell fusion (FUS) genes. The kar mutants fell into two classes that represent distinct events in the yeast karyogamy pathway. Class I mutations blocked congression of the nuclei due to cytoplasmic microtubule defects. In Class II mutants, nuclear congression proceeded and the membranes of apposed nuclei were closely aligned but unfused. In vitro, Class II mutant membranes were defective in a homotypic ER/nuclear membrane fusion assay. We propose that Class II mutants define components of a novel membrane fusion complex which functions during vegetative growth and is recruited for karyogamy. The Rockefeller University Press 1994-08-02 /pmc/articles/PMC2120128/ /pubmed/8051211 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway
title Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway
title_full Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway
title_fullStr Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway
title_full_unstemmed Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway
title_short Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway
title_sort nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120128/
https://www.ncbi.nlm.nih.gov/pubmed/8051211