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Chromokinesin: a DNA-binding, kinesin-like nuclear protein

Microtubule-associated mechanoenzymes have been proposed to play a fundamental role in chromosome movement. We have cloned and characterized the cDNA for a novel protein, named Chromokinesin, that fulfills several of the criteria expected of a mitotic motor. Chromokinesin contains both a kinesin mot...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120390/
https://www.ncbi.nlm.nih.gov/pubmed/7876303
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description Microtubule-associated mechanoenzymes have been proposed to play a fundamental role in chromosome movement. We have cloned and characterized the cDNA for a novel protein, named Chromokinesin, that fulfills several of the criteria expected of a mitotic motor. Chromokinesin contains both a kinesin motor-like domain and an unusual basic-leucine zipper DNA-binding domain. Its mRNA is readily detectable in proliferating cells, but not in postmitotic cells. Immunocytochemical analysis with antibodies directed against the nonconserved COOH-terminal region of Chromokinesin indicates that the protein is localized in the nucleus, and primarily associated with chromosome arms in mitotic cells. These data suggest that Chromokinesin is likely to function as a microtubule-based mitotic motor with DNA as its cargo.
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spelling pubmed-21203902008-05-01 Chromokinesin: a DNA-binding, kinesin-like nuclear protein J Cell Biol Articles Microtubule-associated mechanoenzymes have been proposed to play a fundamental role in chromosome movement. We have cloned and characterized the cDNA for a novel protein, named Chromokinesin, that fulfills several of the criteria expected of a mitotic motor. Chromokinesin contains both a kinesin motor-like domain and an unusual basic-leucine zipper DNA-binding domain. Its mRNA is readily detectable in proliferating cells, but not in postmitotic cells. Immunocytochemical analysis with antibodies directed against the nonconserved COOH-terminal region of Chromokinesin indicates that the protein is localized in the nucleus, and primarily associated with chromosome arms in mitotic cells. These data suggest that Chromokinesin is likely to function as a microtubule-based mitotic motor with DNA as its cargo. The Rockefeller University Press 1995-03-01 /pmc/articles/PMC2120390/ /pubmed/7876303 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Chromokinesin: a DNA-binding, kinesin-like nuclear protein
title Chromokinesin: a DNA-binding, kinesin-like nuclear protein
title_full Chromokinesin: a DNA-binding, kinesin-like nuclear protein
title_fullStr Chromokinesin: a DNA-binding, kinesin-like nuclear protein
title_full_unstemmed Chromokinesin: a DNA-binding, kinesin-like nuclear protein
title_short Chromokinesin: a DNA-binding, kinesin-like nuclear protein
title_sort chromokinesin: a dna-binding, kinesin-like nuclear protein
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120390/
https://www.ncbi.nlm.nih.gov/pubmed/7876303